SDF-1 activity on microvascular endothelial cells: Consequences on angiogenesis in in vitro and in vivo models
SDF-1 activity on microvascular endothelial cells: Consequences on angiogenesis in in vitro and in vivo models
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DOI：
10.1016/s0049-3848(00)00292-9
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发表时间：
2000-09-15
影响因子：
7.5
通讯作者：
Soria, C
中科院分区：
文献类型：
--
作者：
Mirshahi, F;Pourtau, J;Soria, C
趋化因子基质细胞衍生因子 1 (SDF-1) 已被证明参与细胞迁移。由于受体 CXCR-4 在内皮细胞上表达并受到血管生成因子的上调，因此我们被提示研究 SDF-1 对微血管内皮细胞血管生成的影响。这项研究表明，SDF-1 在体外（主要是在三维纤维蛋白凝胶中）诱导血管生成效应。与 SDF-1 一起孵育 10 天后，毛细管形成明显增加。这与微血管内皮细胞（ELISA 和 rt-PCR）轻微增加 VEGF 产生以及有效的趋化作用有关。 SDF-1 还诱导体内血管生成活性，如兔角膜袋模型所示。然而，血管生成位于富含炎症细胞的区域。我们的研究结果表明，这些数据强调了 SDF-1 在血管生成中的潜在作用，因为微血管内皮细胞很大程度上参与了这一过程。 (C) 2000 Elsevier Science Ltd. 保留所有权利。
The chemokine stromal cell-derived factor-1 (SDF-1) has been shown to be involved in cell migration. As the receptor CXCR-4 is expressed on endothelial cells and upregulated by angiogenic factors, we were prompted to study the effect of SDF-1 on angiogenesis in endothelial cells from microvasculature. This study demonstrates that SDF-1 induces an angiogenic effect in vitro, primarily in a tridimensional fibrin gel. The increase in capillary tube formation was evident after a 10-day incubation with SDF-1. This was associated with a mild increase in VEGF production by microvascular endothelial cells (ELISA and rt-PCR) and a potent chemotactic effect. SDF-1 also induced an in vivo angiogenic activity as shown in the model of the rabbit corneal pocket. However, the angiogenesis was located in an area rich in inflammatory cells. The results of our study suggest that these data underline the potential role of SDF-1 in angiogenesis as the microvascular endothelial cells were greatly involved in this process. (C) 2000 Elsevier Science Ltd. All rights reserved.