Evaluation of a novel method for measurement of intracellular calcium ion concentration in fission yeast
Evaluation of a novel method for measurement of intracellular calcium ion concentration in fission yeast
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DOI：
10.2131/jts.42.159
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发表时间：
2017-04-01
影响因子：
2
通讯作者：
Kawasaki, Naohito
中科院分区：
文献类型：
--
作者：
Ogata, Fumihiko;Satoh, Ryosuke;Kawasaki, Naohito
金属和类金属物质在每个细胞隔室中的分布被称为“金属组”。重要的是要阐明潜在的有益或有害的影响，由一个给定的金属或类金属对人类健康的分子机制。因此，我们开发了一种方法来测量细胞内金属离子浓度（特别是细胞内钙离子）在分裂酵母。我们评估了硝酸（HNO 3），酵母酶，和westase处理对裂殖酵母细胞裂解的影响。此外，我们评估了细胞内钙离子浓度的变化，在裂变酵母在治疗与/不米卡芬净。用60%HNO_3处理裂殖酵母，与其它处理方法相比，方法简单，成本低。此外，用电感耦合等离子体原子发射光谱法（ICP-AES）测定了60%HNO3处理后裂殖酵母细胞内钙离子浓度。本研究为测定裂殖酵母胞内钙离子浓度提供了重要信息，有助于阐明钙离子在生物系统中的生理或病理功能。本研究是对金属蛋白质组在生物系统中的作用进行前瞻性研究的第一步。
The distribution of metal and metalloid species in each of the cell compartments is termed as "metallome". It is important to elucidate the molecular mechanism underlying the beneficial or toxic effects exerted by a given metal or metalloid on human health. Therefore, we developed a method to measure intracellular metal ion concentration (particularly, intracellular calcium ion) in fission yeast. We evaluated the effects of nitric acid (HNO3), zymolyase, and westase treatment on cytolysis in fission yeast. Moreover, we evaluated the changes in the intracellular calcium ion concentration in fission yeast in response to treatment with/without micafungin. The fission yeast undergoes lysis when treated with 60% HNO3, which is simpler and cheaper compared to the other treatments. Additionally, the intracellular calcium ion concentration in 60% HNO3-treated fission yeast was determined by inductively coupled plasma atomic emission spectrometry. This study yields significant information pertaining to measurement of the intracellular calcium ion concentration in fission yeast, which is useful for elucidating the physiological or pathological functions of calcium ion in the biological systems. This study is the first step to obtain perspective view on the effect of the metallome in biological systems.