HEPARAN-SULFATE PROTEOGLYCAN EXPRESSION IN NORMAL HUMAN LIVER
HEPARAN-SULFATE PROTEOGLYCAN EXPRESSION IN NORMAL HUMAN LIVER
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DOI：
10.1002/hep.1840210410
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发表时间：
1995-04-01
期刊：
影响因子：
13.5
通讯作者：
DESMET, V
中科院分区：
文献类型：
--
作者：
ROSKAMS, T;MOSHAGE, H;DESMET, V
由于越来越多的证据表明硫酸乙酰肝素蛋白聚糖（HSPG）在受体-生长因子相互作用、细胞-细胞识别系统和细胞-基质粘附过程中作为必需的辅因子，但对其在肝组织中的细胞分布模式和细胞来源知之甚少，我们使用特异于多配体蛋白聚糖1、2、3、4、磷脂酰肌醇蛋白聚糖、和串珠素在正常成人肝组织中的表达。Syndecan 1在肝窦内皮细胞中表达，而门静脉血管内皮细胞为阴性。肝细胞显示膜染色模式的窦状隙和细胞间域。胆管上皮细胞呈基底膜阳性。syndecan 2的免疫反应在间充质细胞中观察到，在咬管周围加重。Syndecan 3显示肝动脉和门静脉内皮细胞、间充质细胞和Ito细胞的强染色。syndecan 4的免疫组化显示肝细胞在其胆小管极的颗粒状染色模式。磷脂酰肌醇蛋白聚糖在汇管区间充质细胞呈弱阳性，在神经束呈明显阳性。串珠素存在于Disse间隙、内皮细胞、胆管和血管周围的基底膜、血管壁和间充质细胞中。这些HSPG在肝脏的不同细胞室中以及在基底膜和Disse空间中的高度差异表达表明，这些蛋白聚糖中的每一种在细胞、基质分子、生长因子和蛋白酶的相互作用中具有特定的功能。
Because increasing evidence implicates heparan sulfate proteoglycans (HSPGs) as essential cofactors in receptor-growth factor interactions, in cell-cell recognition systems, and in cell-matrix adhesion processes and yet Little is known about their cellular distribution pattern and cellular sources in Liver tissue, we used monoclonal antibodies specific for the core proteins of syndecan1, 2, 3, 4, glypican, and perlecan to investigate their immunohistochemical expression in normal adult human Liver biopsy specimens. Syndecan1 was expressed in sinusoidal endothelial cells, whereas the endothelium of the portal tract vessels was negative. Hepatocytes showed a membranous staining pattern of the sinusoidal and intercellular domain. Bile duct epithelial cells showed basolateral membrane positivity. Immunoreactivity for syndecan2 was seen in mesenchymal cells, accentuated around bite ducts. Syndecan3 showed intense staining of hepatic arterial and portal venous endothelial cells, of mesenchymal cells, and of Ito cells. Immunohistochemistry for syndecan4 showed a granular staining pattern of hepatocytes at their bile canalicular pole. Glypican showed weak positivity in portal tract mesenchymal cells and clear positivity in nerve bundles. Perlecan was present in Disse's space, in endothelial cells, in basement membranes surrounding bile ducts and vessels, in vessel walls, and in mesenchymal cells. The highly differential expression of these HSPGs in the different cell compartments of the liver, as well as in basement membranes and in Disse's space, suggests that each of these proteoglycans has a specific function in the interplay of cells, matrix molecules, growth factors, and proteinases.