Infrared microspectroscopy of biochemical response of living cells in microfabricated devices
Infrared microspectroscopy of biochemical response of living cells in microfabricated devices
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DOI：
10.1016/j.vibspec.2010.01.016
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发表时间：
2010-05-26
影响因子：
2.5
通讯作者：
Vaccari, Lisa
中科院分区：
文献类型：
--
作者：
Birarda, Giovanni;Grenci, Gianluca;Vaccari, Lisa
第一个实验证明了新的微加工流体装置的适用性，用于测量活细胞在生理环境中的同步辐射（SR）傅立叶变换红外显微光谱（μ-FTIR）。这些器件是在CaF 2窗口上制造的，使用光致抗蚀剂XARP 3100/10来限定液体单元布局。因此，样品保持器对可见光和红外光都是透明的，坚固耐用，完全可回收，并具有精确的间距。使用原型装置的厚度为9，5和3 μ m，我们研究了U937单核细胞系的机械压缩的响应。FTIR光谱的时间演化。活细胞状态的特征，用于及时监测细胞系统的稳定性。蛋白质的生物合成和脂质代谢的改变变形后已被确定通过监测特定的细胞带比，如酰胺I到酰胺II，酰胺I到脂质，亚甲基到甲基和不对称拉伸的磷酸盐。利用高亮度的同步辐射，单核细胞的化学图谱收集，证明了该设备的多功能性。(C)2010 Elsevier B. V.保留所有权利。
First experiments demonstrating the suitability of novel microfabricated fluidic devices for measuring living cells in physiological environment by synchrotron radiation (SR) Fourier Transform Infrared microspectroscopy (mu-FTIR) are presented. The devices were fabricated on CaF2 windows, using the photoresist XARP 3100/10 to define the liquid cell lay-out. Therefore, the sample holder is transparent to both visible and infrared light, robust, completely recyclable and with a precise spacing. Using prototype devices of thicknesses 9, 5 and 3 mu m, we studied the response of the U937 monocytic cell line to mechanical compression. The temporal evolution of the FTIR spectra. characteristic for the status of living cells, was used to monitor the cellular system stability in time. Protein biosynthesis and lipid metabolism alterations upon deformation have been identified by monitoring specific cell band ratios such as amide I to amide II, amide I to lipids, methylene to methyl and asymmetric to symmetric stretching of phosphates.Taking advantage of the high brilliance of the synchrotron radiation, chemical maps of monocyte cells were collected, demonstrating the versatility of the device. (C) 2010 Elsevier B.V. All rights reserved.