Single Laser to Multiple Optical Fiber Device for Optogenetics-based Epidural Spinal Cord Stimulation
Single Laser to Multiple Optical Fiber Device for Optogenetics-based Epidural Spinal Cord Stimulation
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用于基于光遗传学的硬膜外脊髓刺激的单激光到多光纤装置
DOI：
10.1109/ner.2017.8008327
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Hiroshi Onodera
中科院分区：
文献类型：
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作者：
Shih-Yin Chang;Kazunori Naganuma;Hoshinori Kanazawa;Kenta Takashima;Kuniaki Konishi;Takao Someya;Masaki Sekino;Yasuo Kuniyoshi;Hiroshi Onodera
本文报道了用于光遗传学研究的单激光转多光纤（SLMOF）装置的设计和光学特性。SLMOF的输出端是由210根光纤排列成7×30矩阵制成的光纤束。由半导体激光器（LD）产生的单束激光在光学平台上被精细地控制，以耦合到目标光纤。通过控制LD的功率和光纤之间的快速切换，该器件在设计时空光图案时具有很高的灵活性。我们在体外和体内测试了该设备的光学特性。当使用80 mA的驱动电流时，光以275.4 mW/mm 2的功率从光纤的端部发射。穿透0.2 mm的脊髓切片后，透射分数为18.6%，1 mm后为1.3%。耦合50 ms的重复刺激在10 Hz产生的脊髓表面的温度仅为0.348 K的增加。该系统用于光遗传学研究的能力也通过在表达通道视紫红质-2（ChR 2）的瘫痪大鼠中的硬膜外脊髓刺激诱导的后肢运动来证明。
This paper reports on the design and optical characterization of a single laser to multiple optical fiber (SLMOF) device for optogenetics research. Output end of SLMOF is an optical fiber bundle made by 210 optical fibers arranged into a 7×30 matrix. Single laser beam produced by laser diode (LD) is delicately steered on optical table to be coupled into target optical fiber. By controlling the power of the LD and quickly switching among fibers, this device allows for high flexibility when designing spatiotemporal light patterns. We tested the optical characterization of the device both in vitro and in vivo. Light was emitted from the end of the fiber at a power of 275.4 mW/mm2when a drive current of 80 mA was used. The transmission fraction was 18.6% after penetrating 0.2 mm of spinal cord slice, and 1.3% after 1 mm. Coupling with 50 ms of repetitive stimulation at 10 Hz produced an only 0.348 K temperature increase at the spinal cord surface. The capacity of the system for optogenetics research was also demonstrated by epidural spinal cord stimulation induced hindlimb motion in paralyzed rats expressing channelrhodopsin-2 (ChR2).