GROWTH-HORMONE STIMULATES PROLIFERATION AND DIFFERENTIATION OF NORMAL HUMAN OSTEOBLAST-LIKE CELLS-INVITRO
GROWTH-HORMONE STIMULATES PROLIFERATION AND DIFFERENTIATION OF NORMAL HUMAN OSTEOBLAST-LIKE CELLS-INVITRO
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DOI：
10.1007/bf00298723
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发表时间：
1993-03-01
影响因子：
4.2
通讯作者：
ERIKSEN, EF
中科院分区：
文献类型：
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作者：
KASSEM, M;BLUM, W;ERIKSEN, EF
在这项研究中，我们调查了人类生长激素（hGH）对正常成人成骨细胞样（hOB）细胞的生物学的直接，短期的影响，从骨小梁外植体培养。在亚融合培养物中，hGH以剂量依赖性方式刺激hOB增殖（P < 0.001，n = 15），在10 ng/ml浓度下达到半数最大效应。如溴脱氧尿苷标记所示，这些促有丝分裂作用在24小时内可检测到。在主要含有静止细胞的融合培养物中，hGH增加碱性磷酸酶水平（P < 0.05，n = 10），并在较小程度上增加I型前胶原羧基末端前肽（PICP）水平（P = 0.07，n = 9）。不同细胞株对骨钙素（骨GLa蛋白，BGP）水平的影响差异很大，10种细胞株中只有7种表现出刺激反应（P = 0.16）。我们还研究了hGH对成骨细胞产生胰岛素样生长因子I（IGF-I）和IGF-II以及GH依赖性胰岛素样生长因子结合蛋白3（IGFBP-3）的影响。在基础条件下，人成骨细胞在条件培养基中产生IGF-II和IGFBP-3。当用hGH刺激时，可检测到IGF-II和IGFBP-3（分别为对照的125%和126%）的轻微不显著增加。在基础条件下或用hGH刺激后，在条件培养基中未检测到IGF-I。总之，在这项研究中获得的结果表明，生长激素对人成骨细胞发挥直接的合成代谢作用。
In this study we investigated the direct, short-term effects of human growth hormone (hGH) on the biology of normal adult human osteoblast-like (hOB) cells cultured from trabecular bone explants. In Subconfluent cultures, hGH stimulated hOB proliferation in a dose-dependent fashion (P < 0.001, n = 15) with half-maximal effects at a concentration of 10 ng/ml. These mitogenic effects were detectable within 24 hours as shown by bromodeoxyuridine labeling. In confluent cultures containing mainly quiescent cells, hGH increased levels of alkaline phosphatase (P < 0.05, n = 10) and to a lesser degree levels of procollagen type I carboxyterminal propeptide (PICP) (P = 0.07, n = 9). Effects on osteocalcin (bone GLa protein, BGP) levels were highly variable among different cell strains and only 7 of 10 cell strains showed a stimulatory response (P = 0.16). We also studied the effects of hGH on osteoblastic production of insulin-like growth factor I (IGF-I) and IGF-II as well as the production of GH-dependent, insulin-like growth factor binding protein 3 (IGFBP-3). Under basal conditions, human osteoblasts produced IGF-II and IGFBP-3 in the conditioned medium. When stimulated with hGH, minor insignificant increase in both IGF-II and IGFBP-3 (125% and 126% of control, respectively) were detectable. No IGF-I was detectable in the conditioned medium under basal conditions or after stimulation with hGH. In conclusion, the results obtained in this study suggest that GH exerts direct anabolic effects on human osteoblasts.