Characterization of extreme apical antigens from Toxoplasma gondii.
Characterization of extreme apical antigens from Toxoplasma gondii.
复制标题
弓形虫极端顶端抗原的表征。
作者：
N. Morrissette;V. Bedian;P. Webster;D. Roos
我们分离出 26 种单克隆抗体，它们特异性识别弓形虫的顶端，弓形虫是一种原生动物寄生虫，通过其特化的顶端附着并侵入宿主细胞。定义顶端复合门的独特顶端细胞器被认为与入侵的机械和酶促方面有关。免疫印迹、免疫荧光形态学和免疫金标记定义了这些抗体识别的六类顶端定位抗原。其中三类不溶于去污剂，且定位于锥状体和顶膜的细胞质面，表明它们可能是寄生虫膜细胞骨架的一部分。其余三类用洗涤剂提取并与内膜结合的囊泡（微线体和菱形的上颈）相关。一类微线抗原似乎是细胞周期调节的。在薄的（最近复制的）寄生虫中，这种抗原定位于细胞质，尤其是核周区域，但在较大的寄生虫中位于顶端。
We have isolated 26 monoclonal antibodies which specifically recognize the extreme apex of Toxoplasma gondii, a protozoan parasite which attaches to and invades host cells via its specialized apical end. The unique apical organelles which define the phylum Apicomplexa are thought to be involved in mechanical and enzymatic aspects of invasion. Immunoblots, immunofluorescence morphology, and immunogold labeling define six classes of apically localized antigens recognized by these antibodies. Three of the classes are detergent-insoluble and localize to the conoid and the cytoplasmic face of the apical membrane, suggesting that they may be part of the parasite's membrane cytoskeleton. The remaining three classes extract with detergent and are associated with internal membrane bounded vesicles (micronemes and the upper necks of rhoptries). One class of micronemal antigens appears to be cell cycle regulated. This antigen localizes to the cytoplasm, especially the perinuclear region, in thin (recently replicated) parasites, but is apical in larger parasites.