Carbohydrate expression profile of colorectal cancer cells is relevant to metastatic pattern and prognosis
Carbohydrate expression profile of colorectal cancer cells is relevant to metastatic pattern and prognosis
复制标题
DOI：
10.1023/a:1013879702702
复制
发表时间：
2002-01-01
影响因子：
4
通讯作者：
Kawaguchi, T
中科院分区：
文献类型：
--
作者：
Konno, A;Hoshino, Y;Kawaguchi, T
癌细胞的糖类表达与结直肠癌的转移特性密切相关。在本研究中，我们调查了134例原发灶中结直肠癌细胞糖类表达谱与转移分布模式及预后的相关性。用17种凝集素和3种Lewis相关单抗(MAb)组织化学方法检测糖的表达，并分析其与临床病理参数的关系。结果表明，淋巴侵袭、淋巴结转移和腹膜转移与半乳糖/N-乙酰半乳糖胺残基(Gal/GalNAc)结合的凝集素(Gal/GalNAc)染色有关，如Maclura pomifera(MPA)、花生(PNA)、Helix pomatia(HPA)和Vicia villosa(VVA)。相反，肝转移与鳗鳗凝集素(AAA)、抗Lewis(X)(Lex-2)、抗唾液酸化Lewis(A)(NS19-9)和抗唾液酸化Lewis(X)(FH-6)单抗的染色有关，所有这些单抗都优先与岩藻糖化糖链结合。患者的五年生存率与肿瘤组织中是否表达MPA、HPA、FH-6或NS19-9有关，且两者是独立的预后因素。我们认为癌细胞的糖类表达谱与肿瘤细胞的扩散途径、转移方式以及结直肠癌的预后有关。
Carbohydrate expression of cancer cells is closely related to the metastatic nature of colorectal cancer. In the present study we investigated the relevance of carbohydrate expression profiles of colorectal cancer cells in the primary lesion to metastatic distribution patterns as well as prognosis in 134 cases. Carbohydrate expression was estimated by histochemistry with 17 kinds of lectins and 3 kinds of Lewis-related monoclonal antibodies (MAbs), and correlations between the staining and clinicopathological parameters were examined. The results showed that lymphatic invasion, lymph node metastasis, and peritoneal metastasis correlated with staining with lectins that bind galactose/N-acetylgalactosamine residues (Gal/GalNAc) such as Maclura pomifera (MPA), Arachis hypogaea (PNA), Helix pomatia (HPA), and Vicia villosa (VVA). In contrast, hepatic metastasis correlated with staining with Anguilla anguilla lectin (AAA), anti-Lewis(X) (LEX-2), anti-sialyl Lewis(a) (NS19-9), and anti-sialyl-dimeric Lewis(X) (FH-6) MAbs, all of which bind preferentially to fucosylated carbohydrate chains. The five-year survival rate of patients was related to the staining of cancers with MPA, HPA, FH-6 or NS19-9, and MPA- and FH-6 staining were independent prognostic factors. We conclude that carbohydrate expression profiles of cancer cells are relevant to the route of tumor cell dissemination, metastatic pattern as well as prognosis of colorectal cancer.