Infertile women with isolated polycystic ovaries are deficient in endometrial expression of osteopontin but not αvβ3 integrin during the implantation window
Infertile women with isolated polycystic ovaries are deficient in endometrial expression of osteopontin but not αvβ3 integrin during the implantation window
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DOI：
10.1016/j.fertnstert.2007.11.063
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发表时间：
2009-02-01
影响因子：
6.7
通讯作者：
White, John O.
中科院分区：
文献类型：
--
作者：
DuQuesnay, Renee;Wright, Corrina;White, John O.
目的：探讨孤立性PCO形态的不孕妇女可能存在的子宫内膜缺陷。设计：前瞻性研究。环境：一所拥有试管婴儿（IVF）部门的学术医院。患者：原发不明原因不孕症和孤立性PCO的妇女，有生育能力的妇女和继发于男性因素的妇女。干预(S): 31名妇女（有生育能力和男性因素不育）在月经周期内进行子宫内膜取样。9名有生育能力的妇女和10名有孤立性PCO的不育妇女在LH +7天进行了抽样，根据组织学日期进行了调整。主要观察指标：采用实时定量聚合酶链反应和免疫组织化学检测α v β 3及其配体骨桥蛋白的表达。使用石川细胞系评估骨桥蛋白的体外调节。结果：循环变化显示整合素α v β 3 mRNA在分泌期下降，同时骨桥蛋白mRNA上调。LH +7天免疫组化结果显示，离体PCO组骨桥蛋白表达显著降低，α - v - β 3表达无差异。体外研究证实雌激素可上调骨桥蛋白，雄激素无明显作用。结论：这些结果表明，骨桥蛋白的表达明显减少，在细胞-细胞粘附的重要作用，在着床的窗口不孕妇女分离PCO形态。[j] .中国农业科学（英文版），2009;31(1):489- 498。(C)2009年美国生殖医学学会。)
Objective: To characterize possible endometrial defects in infertile women with isolated PCO morphology.Design: Prospective study. Setting: An academic hospital with an IVF unit.Patient(s): Women with primary unexplained infertility and isolated PCO, fertile women, and women With infertility secondary to male factor.Intervention(S): Thirty-one women (fertile and with male factor infertility) had endometrial sampling across the menstrual cycle. Nine fertile women and 10 infertile women with isolated PCO had sampling on day LH +7, adjusted for histologic dating.Main Outcome Measure(s): Expression of alpha v beta 3 and its ligand, osteopontin, were determined using real-time quantitative polymerase chain reaction and immunohistochemistry. In vitro regulation of osteopontin was assessed using the Ishikawa cell line.Result(s): Cyclic variations revealed a fall in integrin alpha v beta 3 mRNA during the secretory phase with concomitant up-regulation of osteopontin mRNA. Immunohistochemistry on day LH +7 demonstrated a significant reduction in expression of osteopontin in the isolated PCO group with no difference in expression of alpha v beta 3. In vitro studies confirmed Up-regulation of osteopontin by estrogen with no apparent effect of androgen.Conclusion(s): These results demonstrate an apparent reduction of osteopontin expression, important in cell-cell adhesion, during the window of implantation in infertile women with isolated PCO morphology. (Fertil Steril (R) 2009;91:489-99. (C)2009 by American Society for Reproductive Medicine.)