False discovery rate estimation and heterobifunctional cross-linkers
False discovery rate estimation and heterobifunctional cross-linkers
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DOI：
10.1371/journal.pone.0196672
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发表时间：
2018-05-10
期刊：
影响因子：
3.7
通讯作者：
Rappsilber, Juri
中科院分区：
文献类型：
--
作者：
Fischer, Lutz;Rappsilber, Juri
错误发现率(FDR)估计是蛋白质组学的基石，最近已适应于交联/质谱学。在这里，我们证明了异双功能交联剂，虽然在理论上不同于同双功能交联剂，但在实践中不需要单独考虑。我们开发并评估了应用正确的FDR公式使用异双功能交联剂的影响，得出的结论是实际优势最小。因此，可以将单一公式应用于从许多不同的非可切割交联剂产生的数据。
False discovery rate (FDR) estimation is a cornerstone of proteomics that has recently been adapted to cross-linking/mass spectrometry. Here we demonstrate that heterobifunctional cross-linkers, while theoretically different from homobifunctional cross-linkers, need not be considered separately in practice. We develop and then evaluate the impact of applying a correct FDR formula for use of heterobifunctional cross-linkers and conclude that there are minimal practical advantages. Hence a single formula can be applied to data generated from the many different non-cleavable cross-linkers.