Feeding Strategies to Increase Nitrogen Retention and Improve Rumen Fermentation and Rumen Microbial Population in Beef Steers Fed with Tropical Forages
Feeding Strategies to Increase Nitrogen Retention and Improve Rumen Fermentation and Rumen Microbial Population in Beef Steers Fed with Tropical Forages
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DOI：
10.3390/su131810312
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发表时间：
2021-09-01
期刊：
影响因子：
3.9
通讯作者：
Arango, Jacobo
中科院分区：
文献类型：
--
作者：
Durango, Sandra Guisela;Barahona, Rolando;Arango, Jacobo
研究了银合欢（Leucaena leucocephala）和阔叶铁炮草（Tithonia diversifolia）对饲粮中氮（N）排泄量、尿量和瘤胃微生物数量的影响。为了确定干物质摄入量和营养物质排泄量，在由四个时期和四种饮食组成的4x 4拉丁方设计中使用八头阉牛。其中4只在3个放牧时间（T0、T7和T15）进行瘤胃液提取和瘤胃微生物定量。通过外部标记二氧化钛计算草料摄入量。在15个采样日，含上级蛋白质含量的饲料使瘤胃普雷沃氏菌和细菌总数的基因拷贝数增加（p < 0.001）。动物接受的饮食与银合欢和Tithonia的饮食中包含的每日N摄入量为228和113.5g N摄入量d（-1），其中它们排出42%和61%，分别。与以牧草为基础的饮食相比，两种蛋白质饲料的加入增加了每日尿量（9%和7% d（-1））。这项研究表明，包括18%银合欢在牧草为基础的饮食提高干物质的摄入量和氮的保留在热带条件下的瘤牛。
The effect of the inclusion of Leucaena leucocephala and Tithonia diversifolia in Zebu steers receiving a diet based on improved pastures such as Brachiaria decumbens and Brachiaria hybrid cv Cayman on nitrogen (N) excretion, urinary volume and rumen microbial population was evaluated. To determine the dry matter intake and nutrient excretion, eight steers were used in a 4 x 4 Latin square design consisting of four periods and four diets. Four of them were cannulated for ruminal fluid extraction and quantification of ruminal microorganisms in three times of grazing (T0, T7 and T15). Forage intake was calculated through the external marker titanium dioxide. Diet including forages with superior protein content generated an increase in the gene copy numbers of Prevotella ruminicola and total bacteria on 15 sampling day (p < 0.001). Animals receiving diets with the dietary inclusion of Leucaena and Tithonia had daily N intakes of 228 and 113.5g N intake d(-1), of which they excreted 42% and 61%, respectively. Inclusion of both protein forages increased daily urinary volume (9% and 7% d(-1)), with respect to the pasture-based diet. This study revealed that the inclusion of 18% Leucaena in a pasture-based diet improves the dry matter intake and N retention in Zebu steers under tropical conditions.