Temperature-regulated activity of responsive polymer-protein conjugates prepared by grafting-from via RAFT polymerization
Temperature-regulated activity of responsive polymer-protein conjugates prepared by grafting-from via RAFT polymerization
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DOI：
10.1021/ja804495v
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发表时间：
2008-08-27
影响因子：
15
通讯作者：
Sumerlin, Brent S.
中科院分区：
文献类型：
--
作者：
De, Priyadarsi;Li, Ming;Sumerlin, Brent S.
报道了一种制备具有可调生物活性的“智能”聚合物-蛋白质偶联物的简便方法。用可逆加成-断裂链转移（RAFT）试剂和随后在水介质中的室温聚合进行蛋白质修饰导致聚（N-异丙基丙烯酰胺）和模型蛋白质的缀合物。代表通过“R-基团”方法用RAFT试剂固定的聚合物-蛋白质缀合的第一个实例，高分子量和还原稳定的缀合物是可获得的，而无需广泛的纯化或对蛋白质结构的不利影响。从蛋白质表面接枝的链观察到分子量随转化率的增加，证实了聚合的受控性质。固定化聚合物的响应行为促进了缀合物的分离，并且还允许环境调节生物活性。
A facile route to well-defined "smart" polymer-protein conjugates with tunable bioactivity is reported. Protein modification with a reversible addition-fragmentation chain transfer (RAFT) agent and subsequent room temperature polymerization in aqueous media led to conjugates of poly(N-isopropylacrylamide) and a model protein. Representing the first example of polymer-protein conjugation with RAFT agent immobilization via the "R-group" approach, high molecular weight and reductively stable conjugates were accessible without extensive purification or adverse effects on the protein structure. An increase in molecular weight with conversion was observed for the chains grafted from the protein surface, confirming the controlled nature of the polymerization. The responsive behavior of the immobilized polymer facilitated conjugate isolation and also allowed environmental modulation of bioactivity.