Lipogenesis From Ketone Bodies in Perfused Livers From Streptozocin-Induced Diabetic Rats
Lipogenesis From Ketone Bodies in Perfused Livers From Streptozocin-Induced Diabetic Rats
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链佐星诱导的糖尿病大鼠灌注肝脏中酮体的脂肪生成
DOI：
10.2337/diab.37.1.50
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发表时间：
1988
期刊：
影响因子：
7.7
通讯作者：
H. Brunengraber
中科院分区：
文献类型：
--
作者：
L. Freed;G. Endemann;J. F. Tomera;V. Gavino;H. Brunengraber
在正常和链脲佐菌素诱导的糖尿病（STZ-D）大鼠离体肝脏中，用示踪量的3 H2O和（R）-3-羟基[3- 14 C]丁酸酯灌注，测定酮体的产生及其对脂肪生成的贡献。糖尿病使灌流肝和活体大鼠肝中脂肪酸和3-β-羟基甾醇合成的总速率降低80-95%。STZ-D大鼠肝细胞内乙酰乙酰辅酶A合成酶活性轻度降低（17%）。尽管糖尿病和油酸的存在刺激了酮体生成，但在STZ-D大鼠的灌注肝脏中，酮体掺入脂肪酸和甾醇的作用明显受到抑制。在肝脏灌注前用胰岛素治疗大鼠导致生酮和脂肪酸合成率正常化。胰岛素治疗仅使甾醇合成率部分正常化。我们的结论是，在STZ-D，酮症不刺激肝脂肪生成通过乙酰乙酸的胞质激活。
Production of ketone bodies and their contribution to lipogenesis were measured in isolated livers from normal and streptozocin-induced diabetic (STZ-D) rats perfused with tracer amounts of 3H2O and (R)-3-hydroxy[3-14C]butyrate. Diabetes decreased by 80–95% the total rates of fatty acid and 3-β-hydroxysterol synthesis in perfused livers and livers of live rats. The activity of cytosolic acetoacetyl-CoA synthetase was slightly (17%) decreased in livers from STZ-D rats. The incorporation of ketone bodies into fatty acids and sterols was markedly inhibited in perfused livers from STZ-D rats despite the stimulation of ketogenesis by diabetes and the presence of oleate. Treatment of the rats with insulin before liver perfusion led to a normalization of the rates of ketogenesis and fatty acid synthesis. The rates of sterol synthesis were only partially normalized by insulin treatment. We conclude that in STZ-D, ketosis does not stimulate hepatic lipogenesis via cytosolic activation of acetoacetate.
在灌注的大鼠肝脏中丙酮的产生以及丙酮转化为乙酸盐。
DOI：
--
发表时间：
1987
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Gavino,VC;Somma,J;Philbert,L;David,F;Garneau,M;Bélair,J;Brunengraber,H
通讯作者：
Brunengraber,H
预防食欲过盛可以使糖尿病大鼠小肠和肝脏中的 3-羟基-3-甲基戊二酰辅酶 A 还原酶活性正常化。
影响因子：
6.5
作者：
Young,NL;Saudek,CD;Walters,L;Lapeyrolerie,J;Chang,V
通讯作者：
Chang,V
离体灌注大鼠肝脏中酮体的脂肪生成。
DOI：
--
发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Endemann,G;Goetz,PG;Edmond,J;Brunengraber,H
通讯作者：
Brunengraber,H