The cloned gene, Xa21, confers resistance to multiple Xanthomonas oryzae pv. oryzae isolates in transgenic plants.
The cloned gene, Xa21, confers resistance to multiple Xanthomonas oryzae pv. oryzae isolates in transgenic plants.
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DOI：
10.1094/mpmi-9-0850
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发表时间：
1996-12
期刊：
影响因子：
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通讯作者：
Guoyue Wang;Song Wy;Ruan Dl;S. Sideris;P. Ronald
中科院分区：
文献类型：
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作者：
Guoyue Wang;Song Wy;Ruan Dl;S. Sideris;P. Ronald
克隆的水稻基因Xa21赋予水稻对多种病原菌分离物的抗性。转基因植物中的水稻。抗性表型稳定地传递到T1代，并作为单基因座遗传。检测T1后代对32X. oryzaepv.来自8个国家的稻分离物。结果表明，该基因工程菌系和供体菌系对29个菌株表现抗性，对3个菌株表现敏感。两个品系的相同抗性谱表明，多基因家族的单个成员Xa21的存在足以赋予多分离株抗性。本文的研究结果对农作物抗病性的工程化具有重要意义。
The cloned rice gene, Xa21, confers resistance to multiple pathogen isolates of Xanthomonas oryzae pv. oryzae in transgenic plants. The resistance phenotype was stably transmitted to T1 progeny and inherited as a single locus. The T1 progeny were tested for resistance to 32 X. oryzae pv. oryzae isolates from eight countries. Both the engineered line and the donor line showed resistance to 29 isolates and susceptibility to three isolates. The identical resistance spectrum of both lines indicates that the presence of a single member of a multigene family, Xa21, is sufficient to confer multi-isolate resistance. The results presented here have important implications for engineering disease resistance in crop plants.