Microdetermination of plasma ATP and creatine phosphate concentrations with a luminescence biometer.
Microdetermination of plasma ATP and creatine phosphate concentrations with a luminescence biometer.
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使用发光生物计显微测定血浆 ATP 和磷酸肌酸浓度。
DOI：
10.1093/clinchem/23.12.2254
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发表时间：
1977
影响因子：
9.3
通讯作者：
H. Robb
中科院分区：
文献类型：
--
作者：
C. Jabs;W. J. Ferrell;H. Robb
优化了乙醇/水(体积比为96/4)提取血浆中三磷酸腺苷(ATP)和磷酸肌酸(CK)的测定条件。这些程序利用萤火虫荧光素/荧光素酶反应，用杜邦生物仪测量生物发光。用apyrase酶孵育除去血浆中的三磷酸腺苷后，直接测定三磷酸腺苷，通过与肌酸激酶和二磷酸腺苷反应来测定磷酸肌酸。将该方法应用于人、兔和大鼠的血浆，并讨论了可能的临床应用。
We optimized conditions for determination of adenosine-5'-triphosphate (ATP) and creatine phosphate from plasma extracted with ethanol/water (96/4 by vol). The procedures utilize the firefly luciferin/luciferase reaction, the bioluminescence being measured with a Du Pont Biometer. ATP is quantitated directly and creatine phosphate is quantitated by reaction with creatine kinase and ADP, after plasma ATP is removed by incubation with the enzyme apyrase. The method is applied to plasma from humans, rabbits, and rats, and possible clinical applications are discussed.