A high virulence and pathotype diversity of Puccinia striiformis f.sp tritici at its centre of diversity, the Himalayan region of Pakistan
A high virulence and pathotype diversity of Puccinia striiformis f.sp tritici at its centre of diversity, the Himalayan region of Pakistan
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DOI：
10.1007/s10658-014-0461-2
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发表时间：
2014-10-01
影响因子：
1.8
通讯作者：
de Vallavieille-Pope, Claude
中科院分区：
文献类型：
--
作者：
Ali, Sajid;Leconte, Marc;de Vallavieille-Pope, Claude
关于病原菌毒力分布和跨地区多样性的信息对于寄主种质的改进和部署至关重要。小麦条锈菌(Puccinia striiformis f.sp.)对寄主抗性的快速获得Tritici：PST)，因此了解其毒力和致病型多样性是至关重要的。最近的研究表明，病原体可能的起源中心在喜马拉雅地区，巴基斯坦是世界上所有其他人口的最祖先。为了评估巴基斯坦喜马拉雅地区小麦的致病力和致病型多样性状况，从8个地点收集了127份PST侵染小麦样品，利用来自世界套、欧洲套和中国套的36个差异系和9个Avocet YR等位系进行了增殖和病理鉴定。在24个抗条锈病基因中有18个检测到致病力(VR)，在127个菌株中共检测到53个致病型。对巴基斯坦(Vr8)甚至全球水平(Vr5)很少部署的抗性基因发现毒力，而对欧洲常见的Vilmorin 23(Yr3+)巴基斯坦没有毒力。没有一种致病类型在所有地点都是显性的，然而，在所研究的地点没有观察到明显的空间结构。我们的结果表明，巴基斯坦喜马拉雅地区的PST具有很高的毒力和致病型多样性，这与之前提出的有性重组在PST的暂时维持中的潜在作用是一致的。这些信息将有助于寄主抗性基因的改良和部署。
Information on the pathogen virulence profile and diversity across locations is crucial for host germplasm improvement and deployment. The rapid acquisition of virulence to host resistance by the wheat yellow/stripe rust pathogen (Puccinia striiformis f.sp. tritici: PST), makes it crucial to know about its virulence and pathotype diversity. Recent studies have shown the plausible centre of origin of the pathogen in the Himalayan region, with Pakistan being the most ancestral to all other worldwide populations. To assess the status of virulence and pathotype diversity in the Himalayan region of Pakistan, a set of 127 PST infected wheat samples from eight locations were collected, multiplied and pathotyped using a set of 36 differential lines from the world set, European and Chinese sets, and 9 Avocet Yr isolines. Virulence (Vr) was recorded to 18 out of 24 tested yellow rust resistance (Yr) genes, while a total of 53 pathotypes were detected out of 127 isolates tested. Virulence was found to the resistance genes rarely deployed in Pakistan (Vr8) or even worldwide level (Vr5), while virulence to Vilmorin 23 (Yr3+) was absent in Pakistan, which is common in Europe. None of the pathotypes was dominant across all locations, however, no clear spatial structuring was observed for the studied locations. Our results suggested a high virulence and pathotype diversity in line with the previously proposed potential role of sexual recombination in the temporal maintenance of PST in the Himalayan region of Pakistan. This information should be useful in host resistance gene improvement and deployment.