Photocycle populations with femtosecond excitation of crystalline photoactive yellow protein
Photocycle populations with femtosecond excitation of crystalline photoactive yellow protein
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DOI：
10.1016/j.cplett.2016.04.087
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发表时间：
2016-06-16
影响因子：
2.8
通讯作者：
van Thor, Jasper J.
中科院分区：
文献类型：
--
作者：
Hutchison, Christopher D. M.;Kaucikas, Marius;van Thor, Jasper J.
利用飞秒激光脉冲研究了晶体光活性黄蛋白的光周期激发。本工作建立了利用x射线自由电子激光器进行飞秒时间分辨x射线晶体学测量的可行性和合适的光激发条件。闪光光解实验表明，在450nm波长下，脉冲持续时间为133,500和850 fs时，PYP的长寿命“pB”信号状态的光循环产率高达10%。瞬态铅浓度的功率密度依赖于脉冲持续时间，这主要是因为光漂白在GW/mm(2)水平上很突出。(C) 2016年Elsevier B.V.出版
We investigate photocycle excitation of crystalline photoactive yellow protein using femtosecond laser pulses. This work establishes the feasibility and suitable optical excitation conditions to perform femtosecond time resolved X-ray crystallographic measurements using an X-ray free electron laser. Flash photolysis experiments demonstrated photocycle yields of the long-lived 'pB' signalling state of PYP of up to 10% with pulse durations of 130, 500 and 850 fs at 450 nm wavelength. The power density dependence of the transient pB concentration depends strongly on the pulse duration primarily because photobleaching is prominent at the GW/mm(2) level. (C) 2016 Published by Elsevier B.V.