A dual thermoresponsive and antifouling zwitterionic microgel with pH triggered fluorescent "on-off" core.
A dual thermoresponsive and antifouling zwitterionic microgel with pH triggered fluorescent "on-off" core.
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DOI：
10.1016/j.jcis.2020.12.018
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发表时间：
2020-12
影响因子：
9.9
通讯作者：
S. L. Banerjee;Pabitra Saha;Ritabrata Ganguly;Koushik Bhattacharya;Uddhab Kalita;A. Pich;N. Singha
中科院分区：
文献类型：
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作者：
S. L. Banerjee;Pabitra Saha;Ritabrata Ganguly;Koushik Bhattacharya;Uddhab Kalita;A. Pich;N. Singha
通过可逆加成断裂链转移(RAFT)聚合合成了一种独特的、量身定制的两性离子、双温敏和荧光微凝胶探针。采用水包油(O/W)乳液聚合法，以聚羧酸甜菜碱(PCBs)为大分子单体和乳化剂，制备了微凝胶。微凝胶体系中聚N-乙烯基己内酰胺(PNVCL)的存在赋予了体系的热响应性，而罗丹明衍生物作为荧光团的存在使其对体系的pH变化做出响应，在580 nm处(略带橙色)显示荧光发射。通过紫外-可见光谱(UV-Vis)和温度响应性动态光散射(DLS)分析研究了微凝胶的双重热响应性[即存在上临界溶液温度(UCST@12℃)和低临界溶液温度(LcST@33℃)]。电晕交联微凝胶中多氯联苯的存在，对自组装结构的形成以及蛋白质的固定(防污染活性)起着至关重要的作用。以牛血清白蛋白(BSA)为模型蛋白，用UV-Vis光谱研究了其阻垢性能。PH响应性荧光、热响应性以及防污染性能的存在使这种两性离子微凝胶系统成为医疗诊断和药物输送载体的潜在候选者。
A unique, tailor-made, zwitterionic, dual thermoresponsive and fluorescent microgel probe was synthesized via Reversible Addition Fragmentation chain-Transfer (RAFT) polymerization. Microgels were prepared via oil in water (o/w) emulsion polymerization where poly(carboxybetaine) (PCB) acted as a macro-RAFT reagent as well as an emulsifier. The presence of poly(N-vinylcaprolactam) (PNVCL) in the microgel system imparts the thermoresponsiveness to the system and the presence of a rhodamine derivative as fluorophore makes it responsive to pH change of the system by showing a fluorescence emission at 580 nm (reddish orange color). The dual thermoresponsiveness [i.e. the presence of upper critical solution temperature (UCST @ 12 °C) as well as lower critical solution temperature (LCST @ 33 °C)] of the microgels was studied via UV–visible spectroscopy (UV–vis) and temperature responsive dynamic light scattering (DLS) analyses. Presence of the PCB in the corona-crosslinked microgel, played a vital role in the formation of self-assembled structure as well as in protein immobilization (antifouling activity). Antifouling property was studied using UV–vis spectroscopy where bovine serum albumin (BSA) was taken as a model protein. The presence of the pH-responsive fluorescence, thermoresponsiveness as well as antifouling properties makes this zwitterionic microgel system a potential a potential candidate for medical diagnostics and for drug delivery vehicles.