Core RNA polymerase from E-coli induces a major change in the domain arrangement of the σ70 subunit
Core RNA polymerase from E-coli induces a major change in the domain arrangement of the σ70 subunit
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DOI：
10.1016/s1097-2765(00)80313-5
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发表时间：
1999-02-01
期刊：
影响因子：
16
通讯作者：
Heyduk, T
中科院分区：
文献类型：
--
作者：
Callaci, S;Heyduk, E;Heyduk, T
发光共振能量转移测量被用于表明大肠杆菌核心RNA聚合酶的结合诱导了σ(70)亚基中结构域间距离的重大变化。描述核心诱导的σ(70)变化的最简单模型涉及相对于保守区域2，保守区域1移动约20埃以及保守区域4.2移动约15埃。区域1（自身抑制结构域）和区域4.2（DNA结合结构域）的核心诱导移动为核心对σ(70) DNA结合特性的变构调节提供了结构原理，并表明这种调节可能不仅直接涉及σ(70)的自身抑制结构域，而且还可能涉及由核心RNA聚合酶结合诱导的σ(70) DNA结合结构域之间间距的调节。
Luminescence resonance energy transfer measurements were used to show that binding of E. coli core RNA polymerase induced major changes in interdomain distances in the sigma(70) subunit. The simplest model describing core-induced changes in sigma(70) involves a movement of the conserved region 1 by similar to 20 Angstrom and the conserved region 4.2 by similar to 15 Angstrom with respect to conserved region 2. The core-induced movement of region 1 (autoinhibition domain) and region 4.2 (DNA-binding domain) provides structural rationale for allosteric regulation of sigma(70) DNA binding properties by the core and suggests that this regulation may not only involve directly the autoinhibition domain of sigma(70) but also could involve a modulation of spacing between DNA-binding domains of sigma(70) induced by binding of core RNAP.