Elevated expressions of GH/IGF axis genes in triploid crucian carp
Elevated expressions of GH/IGF axis genes in triploid crucian carp
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三倍体鲫鱼GH/IGF轴基因表达升高
DOI：
10.1016/j.ygcen.2012.06.006
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发表时间：
2012-09-01
影响因子：
2.7
通讯作者：
Liu, Yun
中科院分区：
文献类型：
--
作者：
Zhong, Huan;Zhou, Yi;Liu, Yun
生长激素 (GH)、生长激素受体 (GHR) 和胰岛素样生长因子 1 (IGF-1) 是 GH/IGF 轴的关键信号因子，在调节脊椎动物的生长中发挥着至关重要的作用。本研究从三倍体和四倍体鲫鱼中克隆了CH、GHR和IGF-1 cDNA。此外，。对二倍体红鲫鱼、三倍体和四倍体的 mRNA 表达水平进行了表征。逆转录酶PCR表明GH基因仅在垂体中表达，而GHR和IGF-1在所有测试组织中广泛表达。不同季节特征的实时 PCR 研究表明，三倍体在产卵前和产卵季节都有显着较高的研究基因表达。尽管不同温度（22、26和30摄氏度）对二倍体或三倍体中GH、GHR和IGF-1 mRNA表达没有显着影响，但三倍体在每个温度下的表达水平均高于二倍体。禁食1周后：二倍体和三倍体中所有研究基因的表达量均降低，但三倍体中的表达水平高于二倍体。这些结果表明，三倍体中GH/IGF轴基因表达的升高对于三倍体更快的生长速度起着至关重要的作用。 (C) 2012 Elsevier Inc. 保留所有权利。
Growth hormone (GH), growth hormone receptor (GHR) and insulin-like growth factor 1 (IGF-1) are pivotal signaling factors of the GH/IGF axis, which plays a crucial role in regulating growth in vertebrates. In this study, CH, GHR and IGF-1 cDNAs were cloned from triploid and tetraploid crucian carp. In addition,. mRNA expression levels were characterized in diploid red crucian carp, triploids and tetraploids. Reverse transcriptase PCR indicated that GH genes were only expressed in the pituitary, while GHR and IGF-1 were widely expressed in all tested tissues. Real-time PCR study of different seasonal profiles showed that triploids had significantly higher expression of the studied genes during both the prespawning and the spawning season. Although different temperatures (22, 26 and 30 degrees C) showed no significant effects on GH, GHR and IGF-1 mRNA expression in either diploids or triploids, triploids had higher expression levels than diploids at each temperature. After 1 week of fasting:the expression of all studied genes was reduced in both diploids and triploids, while the expressions levels were higher in triploids than in diploids. These results suggest that the elevated expression of GH/IGF axis genes in triploids plays a crucial role in the faster growth rate of triploids. (C) 2012 Elsevier Inc. All rights reserved.