Alcohol Dehydrogenase Type 3 (ADH3) and the Risk of Bladder Cancer
Alcohol Dehydrogenase Type 3 (ADH3) and the Risk of Bladder Cancer
复制标题
3 型酒精脱氢酶 (ADH3) 与膀胱癌的风险
作者：
B.A.C. van Dijk;A. C. V. Houwelingen;J. Witjes;J. Schalken;L. Kiemeney
目的：乙醇脱氢酶(ADH)多态酶催化乙醇转化为致癌代谢物乙醛，乙醛部分排入尿液。这项初步研究的目的是确定该基因多态性是否可能与膀胱癌有关，以及它是否改变了酒精摄入与膀胱癌之间的关系。方法：在奈梅亨大学医学中心纳入120例膀胱癌患者和133例方便对照。一个自我管理的问卷被用来评估酒精摄入量和潜在的混杂因素。提取外周血DNA，用聚合酶链式反应/限制性片段长度多态性方法检测ADH3基因分型。将携带ADH3型γ1γ1的人与其他携带ADH3型的人进行比较。中度以上饮酒者(每周14杯酒)与中度饮酒者进行比较。用Logistic回归分析计算优势比(OR)和95%可信区间。结果：校正性别、年龄和吸烟因素后，ADH3基因和饮酒的OR值分别为2.10(1.05~4.22)和1.21(0.60~2.44)。携带“高风险”(γ1γ1)ADH3基因的中度饮酒者患膀胱癌的风险似乎是携带“低风险”(γ1γ2或γ2γ2)基因的中度饮酒者的三倍。令人惊讶的是，中度以上饮酒者的低风险基因(1.95；95%CI：0.85-4.48)和高危基因(2.18；95%CI：0.82-5.77)的OR值几乎相似，表明没有相互作用。结论：ADH3基因可能是膀胱癌的危险因素。尽管携带γ1γ1基因的适度饮酒者的风险似乎最高，但我们没有得到明确的迹象表明ADH3基因改变了酒精摄入和膀胱癌之间的关系。
Objectives: The polymorphic enzyme alcohol dehydrogenase (ADH) catalyses the conversion of ethanol into the carcinogenic metabolite acetaldehyde which is partly excreted into the urine. Objectives of this pilot study are to determine whether this polymorphism may be related to bladder cancer and whether it modifies the relation between alcohol intake and bladder cancer. Methods: 120 bladder cancer patients and 133 convenience controls were recruited at the University Medical Centre Nijmegen. A self–administered questionnaire was used to assess alcohol intake and potential confounders. DNA was isolated from peripheral blood, and ADH3 genotype was determined using PCR/RFLP. People with the ADH3 genotype γ1γ1 were compared to people with other ADH3 genotypes. Above moderate drinkers (>14 glasses of alcohol per week) were compared to moderate drinkers. Odds ratios (ORs) and 95% confidence intervals were computed using logistic regression analyses. Results: After correction for sex, age and smoking, ORs for ADH3 genotype and alcohol intake were 2.10 (1.05–4.22) and 1.21 (0.60–2.44), respectively. Moderate drinkers with the ‘high–risk’ (γ1γ1) ADH3 genotype appeared to have a threefold higher risk of bladder cancer compared to moderate drinkers with a ‘low–risk’ (γ1γ2 or γ2γ2) genotype. Surprisingly, the ORs for above moderate drinkers with the low–risk genotype (1.95; 95% CI: 0.85–4.48) and with the high–risk genotype (2.18; 95% CI: 0.82–5.77) were almost similar, suggesting no interaction. Conclusions: ADH3 genotype is a possible risk factor for bladder cancer. Although moderate drinkers with the γ1γ1 genotype seem to have the highest risk, we did not get a clear indication that ADH3 genotype modifies the relationship between alcohol intake and bladder cancer.
人类 I 类乙醇脱氢酶基因簇：三个基因串联组织在基因组的 80 kb 长片段中。
影响因子：
4.4
作者：
Yasunami,M;Kikuchi,I;Sarapata,D;Yoshida,A
通讯作者：
Yoshida,A