Increased permeability of the glomerular basement membrane to ferritin after removal of glycosaminoglycans (heparan sulfate) by enzyme digestion.
Increased permeability of the glomerular basement membrane to ferritin after removal of glycosaminoglycans (heparan sulfate) by enzyme digestion.
复制标题
通过酶消化去除糖胺聚糖（硫酸乙酰肝素）后，肾小球基底膜对铁蛋白的渗透性增加。
DOI：
10.1083/jcb.86.2.688
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发表时间：
1980-08
影响因子：
7.8
通讯作者：
Farquhar, M G
中科院分区：
文献类型：
--
作者：
Kanwar, Y S;Linker, A;Farquhar, M G
用糖胺聚糖降解酶原位消化肾小球基底膜（GBM’s），以评估去除糖胺聚糖（GAG）对肾小球基底膜对天然铁蛋白（NF）通透性的影响。肾脏先用酶溶液灌注消化，再用NF灌注消化。在仅用缓冲液处理的对照组中，在毛细血管腔中可见高浓度的NF，但示踪剂没有渗透到GBM内膜（LRI）以外的任何程度，很少或没有NF到达尿腔。肾灌注透明质酸链霉菌酶（去除透明质酸）和软骨素酶- abc（去除透明质酸、硫酸软骨素4-和硫酸软骨素6-和硫酸皮肤素，但不去除硫酸肝素）的结果与对照组相同。在肝素酶消化的肾脏中（可去除包括硫酸肝素在内的大部分GAG）， NF大量穿透GBM并到达尿腔。示踪分子在GBM的层状致密层（LD）和层状外稀层（LRE）中数量增加。在用阳离子化铁蛋白（CF）灌注的对照肾脏中，CF与富硫酸肝素位点结合，先前在稀有层中证实；然而，在肝素酶消化的GBM中未见CF结合，证实酶处理已去除该位点。结果表明，去除硫酸肝素（而不是其他GAG）导致GBM对NF的渗透性急剧增加。
Glomerular basement membranes (GBM's) were subjected to digestion in situ with glycosaminoglycan-degrading enzymes to assess the effect of removing glycosaminoglycans (GAG) on the permeability of the GBM to native ferritin (NF). Kidneys were digested by perfusion with enzyme solutions followed by perfusion with NF. In controls treated with buffer alone, NF was seen in high concentration in the capillary lumina, but the tracer did not penetrate to any extent beyond the lamina rara interna (LRI) of the GBM, and litte or no NF reached the urinary spaces. Findings in kidneys perfused with Streptomyces hyaluronidase (removes hyaluronic acid) and chondroitinase-ABC (removes hyaluronic acid, chondroitin 4- and 6-sulfates, and dermatan sulfate, but not heparan sulfate) were the same as in controls. In kidneys digested with heparinase (which removes most GAG including heparan sulfate), NF penetrated the GBM in large amounts and reached the urinary spaces. Increased numbers of tracer molecules were found in the lamina densa (LD) and lamina rara externa (LRE) of the GBM. In control kidneys perfused with cationized ferritin (CF), CF bound to heparan- sulfate rich sites demonstrated previously in the laminae rarae; however, no CF binding was seen in heparinase-digested GBM's, confirming that the sites had been removed by the enzyme treatment. The results demonstrated that removal of heparan sulfate (but not other GAG) leads to a dramatic increase in the permeability of the GBM to NF.