Identification of the novel bioactive peptides dRYamide-1 and dRYamide-2, ligands for a neuropeptide Y-like receptor in Drosophila
Identification of the novel bioactive peptides dRYamide-1 and dRYamide-2, ligands for a neuropeptide Y-like receptor in Drosophila
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DOI：
10.1016/j.bbrc.2011.06.081
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发表时间：
2011-07-15
影响因子：
3.1
通讯作者：
Kojima, Masayasu
中科院分区：
文献类型：
--
作者：
Ida, Takanori;Takahashi, Tomoko;Kojima, Masayasu
许多生物活性肽参与调节广泛的动物行为，包括食物消耗。脊椎动物神经肽Y（NPY）是一种有效的食欲行为刺激剂。最近，果蝇神经肽F（dNPF）和短NPF（sNPF），果蝇同源的脊椎动物神经肽Y，被确定为NPF的功能，在这种昆虫的摄食行为。Dm-NPFR 1和NPFR 76 F分别是dNPF和sNPF的受体;两种受体都是G蛋白偶联受体（GPCR）。另一个GPCR（CG 5811; NepYR）在果蝇中被鉴定为神经肽Y样受体。在此，我们鉴定了CG 5811的2个配体，dRYamide-1和dRYamide-2。这两种肽均来源于相同的前体（CG 40733），与已知的生物活性肽没有显著的结构相似性。dRYamides的C-末端序列RYamide与NPY家族肽相同;另一方面，dNPF和sNPF具有C-末端RFamide。当给予绿头苍蝇时，dRYamide-1抑制进食动机。我们认为dRYamides与脊椎动物中的NPY家族有关，类似于dNPF和sNPF。(C)2011 Elsevier Inc. All rights reserved.
A number of bioactive peptides are involved in regulating a wide range of animal behaviors, including food consumption. Vertebrate neuropeptide Y (NPY) is a potent stimulator of appetitive behavior. Recently, Drosophila neuropeptide F (dNPF) and short NPF (sNPF), the Drosophila homologs of the vertebrate NPY, were identified to characterize the functions of NPFs in the feeding behaviors of this insect. Dm-NPFR1 and NPFR76F are the receptors for dNPF and sNPF, respectively; both receptors are G protein-coupled receptors (GPCRs). Another GPCR (CG5811; NepYR) was indentified in Drosophila as a neuropeptide Y-like receptor. Here, we identified 2 ligands of CG5811, dRYamide-1 and dRYamide-2. Both peptides are derived from the same precursor (CG40733) and have no significant structural similarities to known bioactive peptides. The C-terminal sequence RYamide of dRYamides is identical to that of NPY family peptides; on the other hand, dNPF and sNPF have C-terminal RFamide. When administered to blowflies, dRYamide-1 suppressed feeding motivation. We propose that dRYamides are related to the NPY family in vertebrates, similar to dNPF and sNPF. (C) 2011 Elsevier Inc. All rights reserved.