WHOLE-MOUNT AUTORADIOGRAPHY STUDY OF DNA SYNTHETIC ACTIVITY DURING POSTNATAL-DEVELOPMENT AND ANDROGEN-INDUCED REGENERATION IN THE MOUSE PROSTATE
WHOLE-MOUNT AUTORADIOGRAPHY STUDY OF DNA SYNTHETIC ACTIVITY DURING POSTNATAL-DEVELOPMENT AND ANDROGEN-INDUCED REGENERATION IN THE MOUSE PROSTATE
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DOI：
10.1095/biolreprod34.5.985
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发表时间：
1986-06-01
影响因子：
3.6
通讯作者：
BRODY, JR
中科院分区：
文献类型：
--
作者：
SUGIMURA, Y;CUNHA, GR;BRODY, JR
将不同年龄和去势后不同时间间隔的小鼠前列腺前叶和背外侧叶（VP和DLP）显微切割成二维阵列，并与14 C-胸苷体外孵育。将标记的全封片标本固定并干燥在载玻片上，浸入照相乳剂中，并进行放射自显影处理。VP和DLP中DNA合成活性的形态学模式相似。在出生后早期（出生后10-15天），DNA合成活性在远端导管尖端（靠近包膜）最高，在近端导管（靠近尿道）相当低。在30日龄时，DNA合成几乎完全局限于远端导管，在近端导管区域标记极低。在完整或去势的成年前列腺中，整个腺体几乎不存在DNA合成，但在导管尖端仍观察到银颗粒。在雄激素诱导的前列腺再生过程中，DNA合成仅在TP给药后24小时的远端导管中检测到。标记强度在TP处理的第三天达到最大值，在远端和近端导管区域，此后，它消退为局限于远端导管的局部标记。这些结果表明，DNA合成活性的水平在前列腺内的区域基础上变化很大。解释前列腺内DNA合成活性的这种异质性是理解前列腺生长和发育的雄激素调节机制的基础。
Ventral and dorsolateral prostatic lobes (VP and DLP), obtained from mice at different ages and at different intervals after castration or treatment of castrated males with testosterone propionate (TP), were microdissected into two-dimensional arrays and incubated in vitro with 14C-thymidine. Labeled whole-mount specimens were fixed and dried onto glass slides, dipped into photographic emulsion, and processed autoradiographically. The morphological pattern of DNA synthetic activity was similar in the VP and DLP. During early postnatal periods (10-15 days after birth), DNA synthetic activity was highest at the distal ductal tips (near the capsule) and considerably lower in proximal ducts (near the urethra). At 30 days of age, DNA synthesis was almost totally confined to the distal ducts, with exceedingly low labeling in the proximal ductal areas. In the prostate of the intact or castrated adult, DNA synthesis was nearly absent throughout the gland, but silver grains were still observed on the ductal tips. During androgen-induced prostatic regeneration, DNA synthesis was detectable only in distal ducts 24 h after TP was administered. Labeling intensity reached a maximum on the third day of TP treatment in both distal and proximal ductal areas, thereafter, it subsided to focal labeling confined mostly to distal ducts. These results demonstrate that levels of DNA synthetic activity vary considerably within the prostate on a regional basis. Explanation of this heterogeneity in DNA synthetic activity within the prostate gland is fundamental to understanding the mechanism of androgenic regulation of prostatic growth and development.