TRANSFORMATION OF YEAST BY A REPLICATING HYBRID PLASMID
TRANSFORMATION OF YEAST BY A REPLICATING HYBRID PLASMID
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DOI：
10.1038/275104a0
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发表时间：
1978-01-01
期刊：
影响因子：
64.8
通讯作者：
BEGGS, JD
中科院分区：
文献类型：
--
作者：
BEGGS, JD
构建嵌合质粒，其含有酵母质粒和与pMB 9连接的酵母核DNA片段，pMB 9是来自大肠杆菌的ColEl质粒的衍生物。分离出两种质粒，它们与E.杆菌这些质粒被用来开发一种方法，用于高频率地将酿酒酵母的leu2菌株转化为Leu+。酵母转化体含有多个质粒拷贝，通过在E.杆菌酵母质粒序列在酵母中繁殖期间分子内重组。
Chimeric plasmids were constructed containing a yeast plasmid and fragments of yeast nuclear DNA linked to pMB9, a derivative of the ColEl plasmid from Escherichia coli. Two plasmids were isolated which complement leuB mutations in E. coli. These plasmids were used to develop a method for transforming a leu2 strain of Saccharomyces cerevisiae to Leu+ with high frequency. The yeast transformants contained multiple plasmid copies which were recovered by transformation in E. coli. The yeast plasmid sequence recombined intramolecularly during propagation in yeast.