Ex vivo Methods for Measuring Cardiac Muscle Mechanical Properties.
Ex vivo Methods for Measuring Cardiac Muscle Mechanical Properties.
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DOI：
10.3389/fphys.2020.616996
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发表时间：
2020
影响因子：
4
通讯作者：
Woulfe KC
中科院分区：
文献类型：
--
作者：
Knight WE;Ali HR;Nakano SJ;Wilson CE;Walker LA;Woulfe KC
心血管疾病仍然是美国发病率和死亡率的主要原因，每年有数千篇论文旨在阐明心脏疾病的潜在机制。用于量化心脏性能的方法相当多种多样，每种技术评估心肌机械特性的独特特征。因此，在这篇综述中，我们讨论了目前的体外方法量化心肌性能，强调可以从每种方法中学到什么，以及每种技术如何结合使用，以补充其他更全面的了解心脏功能。重要的是，心脏功能可以在几个不同的水平上进行评估，从整个器官到单个蛋白质-蛋白质相互作用。在这里，我们采取还原论的观点，通常用于测量心脏机械功能的不同方面的方法，从整个心脏的准备工作，并完成与体外运动试验。虽然每种技术在文献中都有单独的详细记录，但非常需要对这些技术进行比较，描述每种技术可以最好地测量的力学参数，以及每种技术固有的优点和缺点。方法。此外，我们将考虑补充技术，以及如何将这些方法结合起来，以提高我们对心脏机械功能的理解。通过介绍这些方法中的每一种，他们的优势和局限性，在一个单一的手稿，这篇综述将有助于心血管生物学家了解现有的文献心脏机械功能，以及设计未来的实验。
Cardiovascular disease continues to be the leading cause of morbidity and mortality in the United States and thousands of manuscripts each year are aimed at elucidating mechanisms underlying cardiac disease. The methods for quantifying cardiac performance are quite varied, with each technique assessing unique features of cardiac muscle mechanical properties. Accordingly, in this review, we discuss current ex vivo methods for quantifying cardiac muscle performance, highlighting what can be learned from each method, and how each technique can be used in conjunction to complement others for a more comprehensive understanding of cardiac function. Importantly, cardiac function can be assessed at several different levels, from the whole organ down to individual protein-protein interactions. Here, we take a reductionist view of methods that are commonly used to measure the distinct aspects of cardiac mechanical function, beginning with whole heart preparations and finishing with the in vitro motility assay. While each of the techniques are individually well-documented in the literature, there is a significant need for a comparison of the techniques, delineating the mechanical parameters that can are best measured with each technique, as well as the strengths and weaknesses inherent to each method. Additionally, we will consider complementary techniques and how these methods can be used in combination to improve our understanding of cardiac mechanical function. By presenting each of these methods, with their strengths and limitations, in a single manuscript, this review will assist cardiovascular biologists in understanding the existing literature on cardiac mechanical function, as well as designing future experiments.
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影响因子：
5.5
作者：
Fukuda, N;Wu, YM;Granzier, H
通讯作者：
Granzier, H
DOI：
10.1085/jgp.200910276
发表时间：
2009-10
期刊：
The Journal of general physiology
影响因子：
--
作者：
Goo S;Joshi P;Sands G;Gerneke D;Taberner A;Dollie Q;LeGrice I;Loiselle D
通讯作者：
Loiselle D
影响因子：
5
作者：
Chung CS;Granzier HL
通讯作者：
Granzier HL
影响因子：
3.4
作者：
Barman, T;Brune, M;Webb, MR
通讯作者：
Webb, MR
影响因子：
5.5
作者：
DETOMBE, PP;TERKEURS, HEDJ
通讯作者：
TERKEURS, HEDJ