ANALYSIS OF THE CYTOKINE PATTERN EXPRESSED IN-SITU IN INHALANT ALLERGEN PATCH TEST REACTIONS OF ATOPIC-DERMATITIS PATIENTS
ANALYSIS OF THE CYTOKINE PATTERN EXPRESSED IN-SITU IN INHALANT ALLERGEN PATCH TEST REACTIONS OF ATOPIC-DERMATITIS PATIENTS
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DOI：
10.1111/1523-1747.ep12321078
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发表时间：
1995-09-01
影响因子：
6.5
通讯作者：
KRUTMANN, J
中科院分区：
文献类型：
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作者：
GREWE, M;WALTHER, S;KRUTMANN, J
在本研究中，我们通过屋尘螨过敏原斑贴试验检测了特应性皮炎患者湿疹反应发展过程中原位表达的细胞因子模式，在24小时屋尘螨过敏原斑贴试验反应中，白细胞介素(IL)-4 mRNA和IL-2 mRNA的表达增加，但干扰素(IFN)- γ mRNA的表达没有增加，与对照皮肤相比，然而，在48小时吸入性过敏原斑贴试验反应中，ifn - γ mRNA和IL-2 mRNA的表达高于对照皮肤的水平，而IL-4 mRNA的表达低于背景水平。这些数据表明，在特应性皮炎患者的吸入性过敏原贴片试验中，细胞因子反应从th -2样转变为th -1样，这种双相模式是吸入性过敏原贴片试验反应所特有的，因为在同一患者的刺激性反应中没有观察到这种情况。细胞因子IL-12可诱导T细胞产生ifn - γ。在本研究中，吸入性过敏原斑贴试验反应中ifn - γ mRNA表达上调之前，IL-12 mRNA的p35亚基表达增加。这些观察结果表明，IL-12表达的增加可能有助于观察到的原位细胞因子分泌模式的转换。需要进一步的研究来确定在特应性湿疹的起始阶段是否也存在类似的双相细胞因子表达模式。
In the present study, we examined the cytokine pattern expressed in situ during the development of eczematous reactions that had been provoked in atopic dermatitis patients by patch testing with house dust mite allergen, In 24-h house dust mite allergen patch test reactions, expression of interleukin (IL)-4 mRNA and IL-2 mRNA increased, but interferon (IFN)-gamma mRNA did not, as compared with control skin, In 48-h inhalant allergen patch test reactions, however, IFN-gamma mRNA and IL-2 mRNA expression were increased above levels observed in control skin, whereas IL-4 mRNA expression was decreased below background levels, These data demonstrate that a switch from a Th-2-like to a Th-1-like cytokine response occurs in inhalant allergen patch tests of atopic dermatitis patients, This biphasic pattern was specific to inhalant allergen patch test reactions, as it was not observed in irritant reactions in the same patient, IFN-gamma production by T cells may be induced by the cytokine IL-12. In the present study, upregulation of IFN-gamma mRNA expression in inhalant allergen patch test reactions was preceded by an increased expression of the p35 subunit of IL-12 mRNA. These observations suggest that increased IL-12 expression may contribute to the observed switch of the in situ cytokine secretion pattern. Further studies are necessary to determine whether a similar biphasic pattern of cytokine expression is also operative in the initiation phase of atopic eczema.