Electron Donor Effects on Bacterial Surface Sulfhydryl Site Concentrations
Electron Donor Effects on Bacterial Surface Sulfhydryl Site Concentrations
复制标题
电子供体对细菌表面巯基位点浓度的影响
DOI：
10.1080/01490451.2020.1842943
复制
发表时间：
2021
影响因子：
2.3
通讯作者：
Fein, Jeremy B.
中科院分区：
文献类型：
--
作者：
Butzen, Margaret L.;Fein, Jeremy B.
在本研究中，我们研究了对细菌表面巯基位点浓度和分布的两个影响：(1)葡萄糖浓度对细菌细胞表面和表面相关细胞外聚合物（EPS）分子之间巯基位点分布的影响；(2)电子供体身份和浓度对细菌生物量巯基位点浓度的影响。在每组实验中，使用电位滴定法测量总位点浓度，使用位点特异性阻断技术确定巯基位点浓度。在去除细胞EPS和不去除细胞EPS的情况下进行测量。在第一组实验中，我们的结果表明，两种革兰氏阳性细菌——枯草芽孢杆菌和地衣芽孢杆菌，以及一种革兰氏阴性细菌——恶臭假单胞菌，它们的EPS分子上的巯基位点浓度都高于细胞表面的浓度，这可能是为了将有毒金属从细胞表面隔离出来。相反，对于革兰氏阴性的希瓦氏菌，细胞表面巯基位点的浓度大于其EPS分子的浓度。oneidensiscan获得通过金属还原代谢能量,从而提高金属的程度绑定到细胞壁通过巯基结合位点的形成可能是更有益的金属毒性的风险。在第二组实验中，在b的生长培养基中增加两种电子供体丙酮酸盐和葡萄糖的浓度。枯草碱增加了巯基位点所代表的总位点的百分比，但另外两个电子供体甘油和富马酸盐的浓度对巯基位点的百分比没有影响。我们的研究结果表明，电子给体的身份和浓度显著影响细菌细胞表面巯基结合位点的形成。此外，我们的研究结果表明，特定电子供体-细菌物种配对的总能量可用性影响细菌细胞产生巯基结合位点的能力，以及这些位点在细胞表面及其相关EPS分子之间的分布。
In this study, we examined two effects on bacterial surface sulfhydryl site concentrations and distributions: (1) the effect of glucose concentration on the distribution of sulfhydryl sites between the bacterial cell surface and surface-associated extracellular polymeric substance (EPS) molecules, and (2) the effect of electron donor identity and concentration on sulfhydryl site concentrations on bacterial biomass. In each set of experiments, the total site concentration was measured using a potentiometric titration approach, and sulfhydryl site concentrations were determined using a site-specific blocking technique. The measurements were conducted with and without the removal of cellular EPS. For the first set of experiments, our results indicate that the two Gram-positive bacterial species,Bacillus subtilis, andBacillus licheniformis, and one of the Gram-negative bacterial species studied,Pseudomonas putida, each have a greater concentration of sulfhydryl sites on their EPS molecules than is present on their cell surfaces, possibly serving to sequester toxic metals away from the cell surface. Conversely, for the Gram-negative bacterial speciesShewanella oneidensis, the concentration of sulfhydryl sites on the cell surface is greater than that on its EPS molecules.S. oneidensiscan gain metabolic energy through metal reduction, and hence enhancing the extent of metal binding to the cell wall through the formation of sulfhydryl binding sites may be more beneficial than the risk of metal toxicity. In the second set of experiments, increasing the concentration of two electron donors, pyruvate and glucose, in the growth medium ofB. subtilisled to an increase in the percentage of total sites represented by sulfhydryl sites, but the concentration of the two other electron donors, glycerol and fumarate, had no effect on the percentage of sulfhydryl sites. Our results indicate that both the identity and the concentration of electron donors significantly influence the formation of sulfhydryl binding sites on bacterial cell surfaces. In addition, our results suggest that the total energy availability of a specific electron donor-bacterial species pairing affects both the ability of bacterial cells to produce sulfhydryl binding sites, and the distribution of those sites between the cell surface and its associated EPS molecules.
影响因子：
3.2
作者：
Dauner, M;Storni, T;Sauer, U
通讯作者：
Sauer, U