Immunodiffusion test for ovine progressive pneumonia.
Immunodiffusion test for ovine progressive pneumonia.
复制标题
绵羊进行性肺炎的免疫扩散试验。
DOI：
--
复制
发表时间：
1977
影响因子：
1
通讯作者：
Laird Ga
中科院分区：
文献类型：
--
作者：
Cutlip Rc;Jackson Ta;Laird Ga
采用琼脂凝胶免疫扩散法检测绵羊进行性肺炎（OPP）病毒沉淀抗体。试验在塑料培养皿中进行，培养皿中含有6ml 1%纯化琼脂和8%氯化钠缓冲液。血清和抗原孔直径为8毫米，在距中心孔3厘米处呈六角形切割。分别在24小时和48小时进行测试。用于测试的可溶性抗原包括每2周从持续感染OPP病毒分离物WLC 1的细胞培养物中取出浓缩的营养培养基。通过检测实验暴露绵羊和适当对照的血清，验证了结果的特异性。实验接种的羊的一些血清形成两条沉淀线。绵羊接触病毒后不久采集的血清和接触病毒后3至4年采集的血清通常只形成一条沉淀线。37只接种了OPP病毒的羔羊，接种后1个月25%的检测结果为阳性，接种后3个月79%的检测结果为阳性，接种后6个月所有检测结果均为阳性。该试验似乎足以检测绵羊暴露于OPP病毒。
An agar gel immunodiffusion test was developed to detect precipitating antibody against ovine progressive pneumonia (OPP) virus. The test was conducted in plastic petri dishes containing 6 ml of 1% purified agar in tris buffer and 8% sodium chloride. Wells for serum and antigen were 8 mm in diameter and were cut in a hexagonal pattern 3 cm from a central well. Tests were read at 24 and 48 hours. Soluble antigen for the test consisted of concentrated nutrient medium removed every 2 weeks from a cell culture persistently infected with isolate WLC 1 of OPP virus. Specificity of results was verified by testing serums from experimentally exposed sheep and appropriate controls. Two lines of precipitate formed with some serums from experimentally inoculated sheep. Serums taken soon after exposure of sheep to the virus and those taken 3 to 4 years after exposure frequently formed only 1 line of precipitate. Of 37 lambs inoculated with OPP virus, 25% of those tested were positive by postinoculation (PI) month 1, 79% of those tested were positive by PI month 3, and all of those tested were positive by PI month 6. The test appears adequate to detect exposure of sheep to OPP virus.