PERICELLULAR COLOCALISATION AND INTERACTIVE PROPERTIES OF TYPE VI COLLAGEN AND PERLECAN IN THE INTERVERTEBRAL DISC
PERICELLULAR COLOCALISATION AND INTERACTIVE PROPERTIES OF TYPE VI COLLAGEN AND PERLECAN IN THE INTERVERTEBRAL DISC
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DOI：
10.22203/ecm.v032a03
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发表时间：
2016-07-01
影响因子：
3.1
通讯作者：
Melrose, J.
中科院分区：
文献类型：
--
作者：
Hayes, A. J.;Shu, C. C.;Melrose, J.
本研究的目的是免疫定位VI型胶原和perlecan，并确定它们在椎间盘（IVD）中的相互作用特性。共聚焦激光扫描显微镜共定位的perlecan与VI型胶原作为IVD细胞的细胞周成分和跨板层交叉桥在羊和鼠的IVD。与野生型相比，硫酸肝素缺乏Hspg2外显子3缺失小鼠IVD中这些交叉桥的丰度明显较低。这种VI型胶原与弹性成分的关联为其在向环形组织传递弹性反冲特性方面的作用提供了线索。在等离子体共振研究中，Perlecan和VI型胶原高度相互作用。perlecan和VI型胶原的细胞周围共定位提供了基质稳定性和细胞-基质通信，使IVD细胞能够感知和响应其生物力学微环境中的扰动。细胞表面的Perlecan在细胞和周围的细胞外基质之间提供粘附界面。弹性微纤维结构调节张力结缔组织的发育和功能。2010年全球疾病负担研究检查了291种疾病，并确定椎间盘退变和相关的腰痛是全球主要的肌肉骨骼疾病，强调了其巨大的社会经济影响和对更有效治疗策略的需求。更好地了解IVD如何实现其独特的生物力学功能特性对此类治疗措施的发展至关重要。
The aim of this study was to immunolocalise type VI collagen and perlecan and determine their interactive properties in the intervertebral disc (IVD). Confocal laser scanning microscopy co-localised perlecan with type VI collagen as pericellular components of IVD cells and translamellar cross-bridges in ovine and murine IVDs. These cross-bridges were significantly less abundant in the heparin sulphate deficient Hspg2 exon 3 null mouse IVD than in wild type. This association of type VI collagen with elastic components provides clues as to its roles in conveying elastic recoil properties to annular tissues. Perlecan and type VI collagen were highly interactive in plasmon resonance studies. Pericellular colocalisation of perlecan and type VI collagen provides matrix stabilisation and cell-matrix communication which allows IVD cells to perceive and respond to perturbations in their biomechanical microenvironment. Perlecan, at the cell surface, provides an adhesive interface between the cell and its surrounding extracellular matrix. Elastic microfibrillar structures regulate tensional connective tissue development and function. The 2010 Global Burden of Disease study examined 291 disorders and identified disc degeneration and associated low back pain as the leading global musculoskeletal disorder emphasising its massive socioeconomic impact and the need for more effective treatment strategies. A greater understanding of how the IVD achieves its unique biomechanical functional properties is of great importance in the development of such therapeutic measures.