A mutation of the CRUMPLED LEAF gene that encodes a protein localized in the outer envelope membrane of plastids affects the pattern of cell division, cell differentiation, and plastid division in Arabidopsis
A mutation of the CRUMPLED LEAF gene that encodes a protein localized in the outer envelope membrane of plastids affects the pattern of cell division, cell differentiation, and plastid division in Arabidopsis
复制标题
DOI：
10.1111/j.1365-313x.2004.02057.x
复制
发表时间：
2004-05-01
期刊：
影响因子：
7.2
通讯作者：
Machida, Y
中科院分区：
文献类型：
--
作者：
Asano, T;Yoshioka, Y;Machida, Y
我们发现了一个新的突变的核编码基因，命名为CRUMPLED叶（CRL），拟南芥，影响所有植物器官的形态建成和质体分裂。组织学分析表明，在具有crl突变的植物中，茎尖分生组织（SAMs）、根尖和胚的细胞分裂平面被扭曲。此外，我们观察到，在crl突变体中，花序茎中皮层和内皮层细胞以及根内皮层细胞的分化模式受到干扰。这些结果表明，在crl突变体中观察到的形态异常是由于异常的细胞分裂和分化。此外，crl突变体细胞中增大的质体数量减少，表明crl中质体分裂受到抑制。CRL基因编码一种新的蛋白质，分子量为30 kDa，定位于质体包膜中。CRL蛋白在多种植物物种中是保守的，包括蕨类植物和蓝细菌，但在其他生物体中不是。这些数据表明CRL蛋白是质体分裂所必需的，并且它在植物细胞分化和细胞分裂平面的调节中也起重要作用。CRL蛋白的一个可能的功能进行了讨论。
We identified a novel mutation of a nuclear-encoded gene, designated as CRUMPLED LEAF (CRL), of Arabidopsis thaliana that affects the morphogenesis of all plant organs and division of plastids. Histological analysis revealed that planes of cell division were distorted in shoot apical meristems (SAMs), root tips, and embryos in plants that possess the crl mutation. Furthermore, we observed that differentiation patterns of cortex and endodermis cells in inflorescence stems and root endodermis cells were disturbed in the crl mutant. These results suggest that morphological abnormalities observed in the crl mutant were because of aberrant cell division and differentiation. In addition, cells of the crl mutant contained a reduced number of enlarged plastids, indicating that the division of plastids was inhibited in the crl. The CRL gene encodes a novel protein with a molecular mass of 30 kDa that is localized in the plastid envelope. The CRL protein is conserved in various plant species, including a fern, and in cyanobacteria, but not in other organisms. These data suggest that the CRL protein is required for plastid division, and it also plays an important role in cell differentiation and the regulation of the cell division plane in plants. A possible function of the CRL protein is discussed.