Immunization of rams against human recombinant inhibin alpha-subunit delays, augments, and extends season-related increase in blood gonadotropin levels.
Immunization of rams against human recombinant inhibin alpha-subunit delays, augments, and extends season-related increase in blood gonadotropin levels.
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公羊针对人重组抑制素α亚基的免疫延迟、增强和延长了血液促性腺激素水平与季节相关的增加。
DOI：
10.1095/biolreprod42.1.81
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发表时间：
1990
影响因子：
3.6
通讯作者：
Bardin,CW
中科院分区：
文献类型：
--
作者：
Voglmayr,JK;Mizumachi,M;Washington,DW;Chen,CL;Bardin,CW
成年萨福克公羊在80天的时间内针对人重组人白蛋白α-亚基免疫4次。每隔一周采集一次血样，通过放射免疫法测定血清促卵泡激素（FSH）、促黄体生成素（LH）和睾酮水平。结果表明，免疫公羊中促性腺激素水平的季节性升高延迟1 - 2周，并且在这些动物中，其比溶剂处理对照组更明显和延长。对照组公羊的循环睾酮峰值高于免疫组动物。在给药后30天内，每只免疫动物的抗血清结合125 I标记的抗凝血酶α亚基的能力显著增加，尽管在4只免疫公羊中仅1只用大鼠垂体细胞培养生物测定法检测到中和抗体。附睾精子储备往往是更大的免疫比对照组动物。这些结果表明，在繁殖季节，Escherichin控制FSH的释放，从而调节生精活性;它也可能通过对Leydig细胞的局部作用对睾丸功能产生影响，如血清睾酮谱的变化和对Escherichin α-亚基免疫的公羊血清LH水平升高所证明的。
Adult Suffolk rams were immunized four times against the human recombinant inhibin α-subunit over a period of 80 days. Blood samples were collected at weekly intervals and serum levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH), and testosterone were determined by radioimmunoassay procedures. The results show that season-related elevations of gonadotropin levels in immunized rams was delayed by 1 – 2 wk and, in these animals, it was more pronounced and extended than in vehicle-treated controls. Peaks of circulating testosterone were higher in control rams than in immunized animals. The capacity of the antisera to bind125I-labeled inhibin α-subunit increased significantly in each immunized animal within 30 days of treatment, even though neutralizing antibodies were detected with a rat pituitary cell culture bioassay in only one of the four immunized rams. Epididymal sperm reserves tended to be greater in immunized than in control animals. These results show that inhibin controls the release of FSH during the breeding season, thereby regulating spermatogenic activity; it may also exert its effect on testicular function by a local effect on Leydig cells, as evidenced by changes in serum testosterone profiles and increased serum LH levels in rams immunized against the inhibin α-subunit.