Selective accumulation of differentiated CD8+ T cells specific for respiratory viruses in the human lung.
Selective accumulation of differentiated CD8+ T cells specific for respiratory viruses in the human lung.
复制标题
针对人肺中呼吸道病毒的分化CD8+ T细胞的选择性积累。
DOI：
10.1084/jem.20051365
复制
发表时间：
2005-11-21
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
肺部经常受到病毒的攻击，而常驻的CD 8 + T细胞可能有助于监测这些病原体。为了深入了解人类对呼吸道病毒的局部T细胞免疫，我们在配对分析中确定了肺部驻留的CD 8 + T细胞和外周血CD 8 + T细胞的特异性、表型和功能。与循环相比，肺中流感（FLU）特异性和呼吸道合胞病毒（RSV）特异性CD 8 + T细胞的频率明显更高。这与巨细胞病毒和EB病毒特异性CD 8 + T细胞的平均分布形成对比。值得注意的是，肺驻留的FLU-和RSV-特异性CD 8 + T细胞的相当一部分已经进展到相对晚期的分化表型，这反映在CD 28和CD 27的低表达上。肺来源的FLU-specific CD 8 + T细胞具有低活化要求，因为这些细胞的扩增可以在辅助细胞来源的信号不存在的情况下由同源肽启动。因此，人肺中含有大量分化的FLU和RSV特异性记忆CD 8 + T细胞，这些细胞在再次暴露于病毒时容易扩增。常驻肺T细胞可以提供针对肺病毒感染的即时免疫保护。
The lungs are frequently challenged by viruses, and resident CD8+ T cells likely contribute to the surveillance of these pathogens. To obtain insight into local T cell immunity to respiratory viruses in humans, we determined the specificity, phenotype, and function of lung-residing CD8+ T cells and peripheral blood CD8+ T cells in a paired analysis. The lung contained markedly higher frequencies of influenza (FLU)-specific and respiratory syncytial virus (RSV)-specific CD8+ T cells when compared with the circulation. This contrasted with an equal distribution of cytomegalovirus- and Epstein-Bar virus–specific CD8+ T cells. Noticeably, a substantial fraction of the lung-residing FLU- and RSV-specific CD8+ T cells had progressed to a relatively late differentiation phenotype, reflected by low expression of CD28 and CD27. Lung-derived FLU-specific CD8+ T cells had low activation requirements, as expansion of these cells could be initiated by cognate peptide in the absence of helper cell–derived signals. Thus, the human lung contains high numbers of differentiated FLU- and RSV-specific memory CD8+ T cells that can readily expand upon reexposure to virus. Resident lung T cells may provide immediate immunological protection against pulmonary virus infections.