The pyrethroid knock-down resistance gene in the Anopheles gambiae complex in Mali and further indication of incipient speciation within An. gambiae s.s.
The pyrethroid knock-down resistance gene in the Anopheles gambiae complex in Mali and further indication of incipient speciation within An. gambiae s.s.
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DOI：
10.1046/j.1365-2583.2003.00407.x
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发表时间：
2003-06-01
影响因子：
2.6
通讯作者：
Coluzzi, M
中科院分区：
文献类型：
--
作者：
Fanello, C;Petrarca, V;Coluzzi, M
在马里，冈比亚按蚊复合体由An. arabiensis和Mopti、Savanna和巴马科染色体型。冈比亚有限公司以前的染色体数据表明，这些形式之间完全生殖隔离。rDNA区域的序列分析导致两种分子形式的An的特征。gambiae，命名为M-型和S-型，在马里分别对应于莫普提和萨凡纳/巴马科，而到目前为止还没有显示萨凡纳和巴马科之间的任何分子差异。安的人口结构。冈比亚公司在马里巴马科和锡卡索地区的三个村庄进行了分析，并计算了击倒抗性（kdr）型拟除虫菊酯抗性的频率。结果表明，kdr等位基因仅与草原型种群相关，在巴马科、莫普提和安的同域和同步种群中不存在。阿拉伯的。这是巴马科和萨凡纳染色体形式之间基因流动障碍的第一个分子迹象。此外，对1987年在巴马科地区采集的样本进行分析表明，当时在草原种群中已经存在kdr等位基因，而且该等位基因的频率从那时起逐渐增加。
In Mali the Anopheles gambiae complex consists of An. arabiensis and Mopti, Savanna and Bamako chromosomal forms of An. gambiae s.s. Previous chromosomal data suggests a complete reproductive isolation among these forms. Sequence analysis of rDNA regions led to the characterization of two molecular forms of An. gambiae , named M-form and S-form, which in Mali correspond to Mopti and to Savanna/Bamako, respectively, while it has failed so far to show any molecular difference between Savanna and Bamako. The population structure of An. gambiae s.l. was analysed in three villages in the Bamako and Sikasso areas of Mali and the frequency of pyrethroid resistance of the knock-down resistance (kdr) type was calculated. The results show that the kdr allele is associated only with the Savanna form populations and absent in sympatric and synchronous populations of Bamako, Mopti and An. arabiensis. This is the first molecular indication of barriers to gene flow between the Bamako and Savanna chromosomal forms. Moreover, analyses of specimens collected in the Bamako area in 1987 show that the kdr allele was already present in the Savanna population at that time, and that the frequency of this allele has gradually increased since then.