Increase of CC chemokine receptor 4-positive cells in the peripheral CD4+ cells in dogs with atopic dermatitis or experimentally sensitized to Japanese cedar pollen
Increase of CC chemokine receptor 4-positive cells in the peripheral CD4+ cells in dogs with atopic dermatitis or experimentally sensitized to Japanese cedar pollen
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DOI：
10.1111/j.1365-2222.2004.02039.x
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发表时间：
2004-09-01
影响因子：
6.1
通讯作者：
Tsujimoto, H
中科院分区：
文献类型：
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作者：
Maeda, S;Ohmori, K;Tsujimoto, H
由于狗经常发生过敏性疾病，与人类相似，狗代表了人类过敏的可能动物模型。在人类特应性皮炎（AD）中，CC趋化因子受体4（CCR 4）已被证明在AD的过敏性炎症的发展中起重要作用;但是，在此情况下，CCR 4在犬过敏反应中的作用尚不清楚。目的探讨CCR 4在犬外周血CD 4（+）细胞在狗，AD和日本雪松花粉实验致敏。材料和方法外周血单个核细胞（PBMC）分离自17只狗与AD。采用流式细胞仪检测CCR 4（+）细胞占外周血CD 4（+）细胞的比例（CCR 4/CD 4），并与10只健康犬进行比较。结果AD犬CCR 4/CD 4比值为40.3 ± 3.3%，明显高于正常犬（23.6 ± 4.3%）（P < 0.01）。
Background Since dogs frequently develop allergic diseases, similar to those in humans, dogs represent a possible animal model for allergy in humans. In human atopic dermatitis (AD), CC chemokine receptor 4 (CCR4) has been shown to play an important role in the development of allergic inflammation of AD; however, the association between allergic reaction and CCR4 is not well understood in dogs.Objective To examine CCR4 expression in peripheral blood CD4(+) cells in dogs that had AD and were experimentally sensitized with Japanese cedar pollen.Materials and methods Peripheral blood mononuclear cells (PBMCs) were isolated from 17 dogs with AD. The proportion of CCR4(+) cells in peripheral blood CD4(+) cells (CCR4/CD4) was evaluated by flow cytometry and compared with that in 10 healthy dogs. Similarly, in dogs that were experimentally sensitized to Japanese cedar pollen antigen, the proportion of CCR4/CD4 was examined pre- and post-sensitization.Results The proportion of CCR4/CD4 in dogs with AD was 40.3+/-3.3%, which was significantly higher than that in normal dogs (23.6+/-4.3%) (P