INDUCIBLE BINDING OF A FACTOR TO THE C-FOS ENHANCER
INDUCIBLE BINDING OF A FACTOR TO THE C-FOS ENHANCER
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DOI：
10.1016/0092-8674(86)90520-9
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发表时间：
1986-12-05
期刊：
影响因子：
64.5
通讯作者：
ROEDER, RG
中科院分区：
文献类型：
--
作者：
PRYWES, R;ROEDER, RG
我们已经确定了核提取物中的一个因子，它与c-fos增强子结合。用表皮生长因子处理A431细胞导致转录水平的快速增加和该因子与增强子的结合的伴随增加。令人惊讶的是，随着转录的快速降低，增强子结合活性保持升高。此外，虽然HeLa细胞没有表现出可检测到的c-fos基因的转录，但它们含有大量的结合活性，与诱导的A431细胞中的结合活性相当。这些结果表明，c-fos转录的调控不仅仅涉及能够结合增强子的因子水平的增加。最后，在A431细胞中c-fos的转录显着诱导的肿瘤促进剂TPA和钙离子载体A23187，但都没有诱导的进入者结合活性的水平增加。因此，这些药物似乎通过一种不同于表皮生长因子的机制激活c-fos转录。
We have identified a factor in nuclear extracts that binds to the c-fos enhancer. Treatment of A431 cells with epidermal growth factor results in a rapid increase in the level of transcription and a concomitant increase in binding of the factor to the enhancer. Surprisingly, as transcription decreases rapidly, the enhancer-binding activity remains elevated. In addition, although HeLa cells exhibit no detectable transcription of the c-fos gene, they contain significant amounts of binding activity, comparable to those in induced A431 cells. These results suggest that regulation of c-fos transcription involves more than simply an increased level of a factor capable of binding the enhancer. Finally, transcription of c-fos in A431 cells is markedly induced by the tumor promoter TPA and the calcium ionophore A23187, yet neither induced an increased level of the entrancer-binding activity. These agents thus appear to activate c-fos transcription via a mechanism distinct from that used by epidermal growth factors.