A system to impose prescribed homogenous strains on cultured cells.
A system to impose prescribed homogenous strains on cultured cells.
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一种对培养细胞施加规定的同质菌株的系统。
DOI：
10.1152/jappl.2001.91.4.1600
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Bearinger,JP
中科院分区：
文献类型：
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作者：
Waters,CM;Glucksberg,MR;Lautenschlager,EP;Lee,CW;VanMatre,RM;Warp,RJ;Savla,U;Healy,KE;Moran,B;Castner,DG;Bearinger,JP
目前人们对细胞对物理力的反应非常感兴趣，并且已经开发了许多设备来对培养的细胞施加拉伸。许多早期的装置受到不同位置细胞变形的异质性以及特定位置的高度各向异性的限制。因此，我们开发了一种系统，可以在铺有肺上皮细胞的多孔表面处理的有机硅弹性体基底上施加循环、大应变、均匀拉伸。气动驱动机构由四块板组成，每块板都有一个夹具来固定十字形弹性体基板的一个边缘。四个线性轴承以预定角度设置在板之间，确保在整个拉伸周期中主应变比恒定。我们介绍了装置和膜形状的设计、促进细胞粘附的膜表面修饰、应变场的预测和实验测量，以及使用培养的气道上皮细胞的新数据。我们首次提出了循环机械应变的大小与伤口闭合和细胞扩散程度之间的关系。
There is presently significant interest in cellular responses to physical forces, and numerous devices have been developed to apply stretch to cultured cells. Many of the early devices were limited by the heterogeneity of deformation of cells in different locations and by the high degree of anisotropy at a particular location. We have therefore developed a system to impose cyclic, large-strain, homogeneous stretch on a multiwell surface-treated silicone elastomer substrate plated with pulmonary epithelial cells. The pneumatically driven mechanism consists of four plates each with a clamp to fix one edge of the cruciform elastomer substrate. Four linear bearings set at predetermined angles between the plates ensure a constant ratio of principal strains throughout the stretch cycle. We present the design of the device and membrane shape, the surface modifications of the membrane to promote cell adhesion, predicted and experimental measurements of the strain field, and new data using cultured airway epithelial cells. We present for the first time the relationship between the magnitude of cyclic mechanical strain and the extent of wound closure and cell spreading.