Borrelia burgdorferi strain-specific Osp C-mediated immunity in mice
Borrelia burgdorferi strain-specific Osp C-mediated immunity in mice
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DOI：
10.1128/iai.65.11.4661-4667.1997
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发表时间：
1997-11-01
影响因子：
3.1
通讯作者：
Barthold, SW
中科院分区：
文献类型：
--
作者：
Bockenstedt, LK;Hodzic, E;Barthold, SW
针对伯氏疏螺旋体外表面蛋白A、B和C的抗体可以预防莱姆疏螺旋体病动物模型的感染。我们先前已经证明，来自感染伯氏杆菌N40的小鼠的免疫血清也可以预防攻击感染，并在感染小鼠中诱导疾病消退。保护性和疾病调控抗体的靶向抗原目前尚不清楚，但它们不包括OSP A或OSP B。由于OSP C抗体存在于免疫小鼠血清中，因此我们研究了高免血清与重组OSP C(N40)的结合能力，以保护小鼠免受N40螺旋体的攻击感染。在主动免疫和被动免疫研究中，OSP C(N40)抗血清均不能保护小鼠免受培养生物的攻击感染。用重组OSP C(N40)主动免疫的小鼠对硬蜱攻击感染易感，若虫在取食OSP C高免小鼠后仍可感染。相反，用OSP C(PKO)抗血清进行的类似免疫研究防止了对小鼠致病的PKO螺旋体克隆对小鼠的攻击感染。OSP C(N40)和OSP C(PKO)抗血清在体外均显示极低的杀螺活性，免疫荧光研究显示OSP C位于N40和PKO螺旋体外膜下。我们得出结论，OSP C抗体介导的免疫是菌株特异性的，并提出体内螺旋体在OSP C表面表达的差异可能是菌株特异性免疫的原因。
Antibodies to the outer surface proteins (Osps) A, B, and C of the spirochete Borrelia burgdorferi can prevent infection in animal models of Lyme borreliosis. We have previously demonstrated that immune serum from mice infected with B. burgdorferi N40 can also prevent challenge infection and induce disease regression in infected mice. The antigens targeted by protective and disease-modulating antibodies are presently unknown, but they do not include Osp A or Osp B. Because Osp C antibodies are present in immune mouse serum, we investigated the ability of hyperimmune serum to recombinant Osp C (N40) to protect mice against challenge infection with N40 spirochetes. In both active and passive immunization studies, Osp C (N40) antiserum failed to protect mice from challenge infection with cultured organisms. Mice actively immunized with recombinant Osp C (N40) were susceptible to tick-borne challenge infection, and nymphal ticks remained infected after feeding on Osp C-hyperimmunized mice. In contrast, similar immunization studies performed with Osp C (PKo) antiserum prevented challenge infection of mice with a clone of PKo spirochetes pathogenic for mice. Both Osp C (N40) and Osp C (PKo) antisera showed minimal in vitro borreliacidal activity, and immunofluorescence studies localized Osp C beneath the outer membrane of both N40 and PKo spirochetes. We conclude that Osp C antibody-mediated immunity is strain specific and propose that differences in Osp C surface expression by spirochetes in vivo may account for strain-specific immunity.