Analysis of 18FDG PET/CT Imaging as a Tool for Studying Mycobacterium tuberculosis Infection and Treatment in Non-human Primates.
Analysis of 18FDG PET/CT Imaging as a Tool for Studying Mycobacterium tuberculosis Infection and Treatment in Non-human Primates.
复制标题
DOI：
10.3791/56375
复制
发表时间：
2017-09
期刊：
影响因子：
--
通讯作者：
Alexander G. White;Pauline Maiello;M. Coleman;Jaime Tomko;L. Frye;C. Scanga;P. Lin;JoAnne L. Flynn
中科院分区：
文献类型：
--
作者：
Alexander G. White;Pauline Maiello;M. Coleman;Jaime Tomko;L. Frye;C. Scanga;P. Lin;JoAnne L. Flynn
结核分枝杆菌仍然是当今世界第一大传染源。随着抗生素耐药菌株的出现，需要新的临床相关方法来评估疾病过程并筛选潜在的抗生素和疫苗治疗。正电子发射断层扫描/计算机断层扫描 (PET/CT) 已成为研究癌症、阿尔茨海默病和炎症/感染等多种疾病的重要工具。这里概述了一些已用于评估支气管内感染低剂量结核分枝杆菌的食蟹猴的 PET/CT 图像的策略。通过评估 CT 上的病灶大小以及 PET 图像中病灶和淋巴结中 18F-氟脱氧葡萄糖 (FDG) 的摄取，这些描述的方法表明 PET/CT 成像可以预测活动性与潜伏性疾病的未来发展以及从潜伏感染状态重新激活的倾向。此外，通过分析肺部炎症的总体水平，这些方法可以确定药物在临床最相关的现有动物模型中对抗结核分枝杆菌的抗生素功效。这些图像分析方法是对抗这种疾病的最强大的工具，因为它们不仅可以评估感染和药物治疗的许多特征，而且还可以直接转化为用于人类研究的临床环境。
Mycobacterium tuberculosis remains the number one infectious agent in the world today. With the emergence of antibiotic resistant strains, new clinically relevant methods are needed that evaluate the disease process and screen for potential antibiotic and vaccine treatments. Positron Emission Tomography/Computed Tomography (PET/CT) has been established as a valuable tool for studying a number of afflictions such as cancer, Alzheimer's disease, and inflammation/infection. Outlined here are a number of strategies that have been employed to evaluate PET/CT images in cynomolgus macaques that are infected intrabronchially with low doses of M. tuberculosis. Through evaluation of lesion size on CT and uptake of 18F-fluorodeoxyglucose (FDG) in lesions and lymph nodes in PET images, these described methods show that PET/CT imaging can predict future development of active versus latent disease and the propensity for reactivation from a latent state of infection. Additionally, by analyzing the overall level of lung inflammation, these methods determine antibiotic efficacy of drugs against M. tuberculosis in the most clinically relevant existing animal model. These image analysis methods are some of the most powerful tools in the arsenal against this disease as not only can they evaluate a number of characteristics of infection and drug treatment, but they are also directly translatable to a clinical setting for use in human studies.