BARRIER PROPERTIES OF THE GRAM-NEGATIVE CELL-ENVELOPE TOWARDS HIGH-MOLECULAR-WEIGHT POLYHEXAMETHYLENE BIGUANIDES
BARRIER PROPERTIES OF THE GRAM-NEGATIVE CELL-ENVELOPE TOWARDS HIGH-MOLECULAR-WEIGHT POLYHEXAMETHYLENE BIGUANIDES
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DOI：
10.1111/j.1365-2672.1990.tb01552.x
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发表时间：
1990-10-01
期刊：
影响因子：
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通讯作者：
WILKINSON, DE
中科院分区：
文献类型：
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作者：
GILBERT, P;PEMBERTON, D;WILKINSON, DE
本文研究了四种不同分子量组分的多聚双胍类化合物对多种大肠杆菌的抗菌活性。虽然观察到聚合物的活性与聚合数成比例地增加，但活性对分子量的依赖性对原生质球比对全细胞大五倍。这表明，细胞包膜虽然没有赋予对药剂的完全抗性，但确实提供了显著的排斥屏障。对粗糙和深粗糙的脂多糖菌株的活性的比较显示生长抑制活性，但没有杀菌活性，也没有呼吸抑制，在粗糙的菌株中得到增强。摄取研究表明，混合H-和C-型吸附与显着更大的高亲和力结合位点的数量与粗糙比深粗糙脂多糖。在粗糙的菌株中，对细胞的多聚双胍的结合亲和力也增强。结合亲和力，在所有情况下，显着降低镁的存在下，并建议这些杀生物剂的自我促进的吸收机制，通过核心脂多糖促进。
The antimicrobial activities of four discrete molecular weight fractions of polyhexamethylene biguanides towards a number of Escherichia coli strains have been investigated. Whilst activity of the polymers was observed to increase in proportion to polymerization number, the dependence of activity upon molecular weight was five times greater towards sphaeroplasts than towards whole cells. This suggested that the cell envelope, whilst not conferring complete resistance to the agents, did provide a significant exclusion barrier. Comparison of the activities towards rough and deep-rough lipopolysaccharide strains showed growth inhibitory activity, but not bactericidal activity nor respiratory inhibition, to be enhanced in the rough strains. Uptake studies showed mixed H- and C-type adsorption with significantly greater numbers of high-affinity binding sites being associated with rough than deep-rough lipopolysaccharide. The binding affinity of polyhexamethylene biguanides towards cells was also enhanced in the rough strains. Binding affinity was, in all cases, significantly reduced in the presence of magnesium and suggested a mechanism of self-promoted uptake for these biocides, facilitated through core lipopolysaccharide.