MOUSE EMBRYONIC STEM-CELLS AND REPORTER CONSTRUCTS TO DETECT DEVELOPMENTALLY REGULATED GENES
MOUSE EMBRYONIC STEM-CELLS AND REPORTER CONSTRUCTS TO DETECT DEVELOPMENTALLY REGULATED GENES
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DOI：
10.1126/science.2497519
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发表时间：
1989-04-28
期刊：
影响因子：
56.9
通讯作者：
SKARNES, WC
中科院分区：
文献类型：
--
作者：
GOSSLER, A;JOYNER, AL;SKARNES, WC
设计了一种用于鉴定小鼠基因组中在发育过程中以时间和空间受限的方式转录活跃的区域的策略。该方法是基于引入胚胎干细胞的两种类型的lacZ报告构建体，可以激活侧翼小鼠基因组序列。含有lacZ构建体的胚胎干细胞用于产生嵌合小鼠。lacZ表达的发育调节发生在一个高频率。分子克隆的侧翼内源性基因和这些潜在的插入突变到小鼠生殖细胞系中的介绍，应提供一个有效的手段，确定和突变的新基因的重要控制哺乳动物的发展。
A strategy was devised for identifying regions of the mouse genome that are transcriptionally active in a temporally and spatially restricted manner during development. The approach is based on the introduction into embryonic stem cells of two types of lacZ reporter constructs that can be activated by flanking mouse genomic sequences. Embryonic stem cells containing the lacZ constructs were used to produce chimaeric mice. Developmental regulation of lacZ expression occurred at a high frequency. Molecular cloning of the flanking endogenous genes and introduction of these potential insertional mutations into the mouse germ line should provide an efficient means of identifying and mutating novel genes important for the control of mammalian development.