METHYLATED NUCLEOTIDES BLOCK 5'-TERMINUS OF VACCINIA VIRUS MESSENGER-RNA
METHYLATED NUCLEOTIDES BLOCK 5'-TERMINUS OF VACCINIA VIRUS MESSENGER-RNA
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DOI：
10.1073/pnas.72.1.318
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发表时间：
1975-01-01
影响因子：
11.1
通讯作者：
MOSS, B
中科院分区：
文献类型：
--
作者：
WEI, CM;MOSS, B
对牛痘病毒颗粒体外合成的mRNA中甲基化核苷酸的性质和位置的研究揭示了一种不寻常的5 '端结构。焦磷酸基团被7-甲基鸟苷阻断，2 ′-O-甲基腺苷和2 ′-O-甲基鸟苷占据倒数第二个位置的证据被提出。根据该模型，牛痘病毒mRNA的5 '-末端是：7 MeG-5' ppp-5 'GMepNp和7 MeG-5' AMepNp。
Studies on the nature and location of the methylated nucleotides in mRNA synthesized in vitro by vaccinia virus particles revealed an unusual 5'-terminal structure. Evidence that the pyrophosphate group is blocked by 7-methylguanosine and that both 2'-O-methyl-adenosine and 2'-O-methylguanosine occupy penultimate positions was presented. According to this model, the 5'-termini of vaccinia virus mRNAs are: 7MeG-5'ppp-5'GMepNp and 7MeG-5'AMepNp.