Preproenkephalin mRNA expression in developing rat heart and in cultured ventricular cardiac muscle cells.
Preproenkephalin mRNA expression in developing rat heart and in cultured ventricular cardiac muscle cells.
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DOI：
10.1042/bj2580073
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发表时间：
1989-02
期刊：
影响因子：
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通讯作者：
J. Springhorn;W. Claycomb
中科院分区：
文献类型：
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作者：
J. Springhorn;W. Claycomb
先前已报道，心肌组织在成年大鼠的任何组织中具有最高含量的前脑啡肽原（ppEnk）mRNA。我们已经确定它存在于心脏的心室心肌细胞中，并受到发育调节。观察到ppEnk mRNA的表达在出生后发育的前2周内较低，并在第3周期间大幅降低。到第4周表达再次降低，但到成年期（约10天）。3个月），达到最大值。ppEnk mRNA在从新生大鼠和成年大鼠制备的原代心肌细胞培养物中积极表达。它的稳态含量在细胞培养物中观察到增加环AMP和3-异丁基-1-甲基黄嘌呤。佛波酯佛波醇12-肉豆蔻酸酯13-乙酸酯引起瞬时效应（即在4小时观察到增加，到24小时恢复到对照值）。我们推测脑啡肽可能在新生心肌细胞分化和终末分化的成人心肌细胞中发挥多功能作用。我们证明，在这项研究中采用的原代培养系统将是有用的模型，探索ppEnk mRNA在心脏中的转录和翻译调控。
Heart muscle tissue has previously been reported to have the highest content of preproenkephalin (ppEnk) mRNA of any tissue in the adult rat. We have determined that it is present in the ventricular cardiac muscle cells of the heart and is developmentally regulated. The expression of ppEnk mRNA was observed to be low throughout the first 2 weeks of postnatal development and decreases substantially during week 3. Expression was again low by week 4, but by adulthood (approx. 3 months), it reached a maximum. ppEnk mRNA was actively expressed in primary cardiac muscle cell cultures prepared from both neonatal and adult rats. Its steady-state content in cell cultures was observed to be increased by cyclic AMP and 3-isobutyl-1-methylxanthine. The phorbol ester phorbol 12-myristate 13-acetate elicited a transient effect (i.e. an increase was observed at 4 h and a return to control values by 24 h). We speculate that enkephalin may play a multi-functional role in the differentiation of neonatal cardiac muscle cells and in the terminally differentiated adult heart cell. We demonstrate that the primary culture systems employed in this study will be useful models with which to explore both transcriptional and translational regulation of ppEnk mRNA in the heart.