REGULATION OF STEROIDOGENESIS IN CULTURED PORCINE THECA CELLS BY GROWTH-FACTORS
REGULATION OF STEROIDOGENESIS IN CULTURED PORCINE THECA CELLS BY GROWTH-FACTORS
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DOI：
10.1210/endo-125-1-321
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发表时间：
1989-07-01
期刊：
影响因子：
4.8
通讯作者：
TONETTA, SA
中科院分区：
文献类型：
--
作者：
CAUBO, B;DEVINNA, RS;TONETTA, SA
生长因子[胰岛素样生长因子（IGF-I, IGF-II），转化生长因子- β。（tgf . β），表皮生长因子（EGF）]，在卵巢中发现并已知改变颗粒功能，评估其调节猪鞘类固醇生成的能力。大猪卵泡（8-10 mm）的卵泡膜细胞镀5倍。105个细胞/ml。在无血清的M199中，增加剂量的生长因子：IGF-I (0.1-50 ng/ml), IGF-II (0.5-200 ng/ml), EGF (0.021-100 ng/ml), tgf . β。（0.001-40 ng/ml）或胰岛素(0.01-50 .mu。g/ml)，含或不含人CG [(hCG)；20 ng/ml]，孵育72 h。采用RIA法测定培养基中甾体激素水平。胰岛素增加了基础和促性腺激素诱导的雄烯二酮、孕酮、雌二醇和睾酮的分泌（P < 0.05）。在最高剂量下，IGF-I增加了基础和hcg诱导的孕酮和雄烯二酮的分泌（P < 0.05），但对雌二醇和睾酮的分泌没有影响。在最高剂量下，IGF-II增加了（P < 0.05）硬膜甾体生成，但仅在给药hCG之后。相反，tgf。增加了雌二醇的基础分泌量和促性腺激素诱导的雌二醇分泌量（P < 0.05），抑制了鞘内黄体酮、雄烯二酮和睾酮的分泌。EGF不改变黄体酮、雄烯二酮和睾酮的分泌，但显著（P < 0.05）抑制基础和hcg刺激的雌二醇分泌。总之，胰岛素，igf - 1, igf - 2， EGF和tgf。可调节猪乳膜细胞的类固醇生成。
Growth factors [insulin-like growth factors (IGF-I, IGF-II), transforming growth factor-.beta. (TGF.beta.), epidermal growth factors (EGF)], found in the ovary and known to alter granulosal function, were assessed for their ability to modulate porcine thecal steroidogenesis. Theca cells from large porcine follicles (8-10 mm) were plated (5 .times. 105 cells/ml .cntdot. well) in serum-free M199, treated with increasing doses of growth factors: IGF-I (0.1-50 ng/ml), IGF-II (0.5-200 ng/ml), EGF (0.021-100 ng/ml), TGF.beta. (0.001-40 ng/ml), or insulin (0.01-50 .mu.g/ml), with or without human CG [(hCG); 20 ng/ml], and incubated for 72 h. Levels of steroids in media were determined by RIA. Insulin increased (P < 0.05) basal and gonadotropin-induced secretion of androstenedione, progesterone, estradiol, and testosterone. IGF-I increased (P < 0.05) the basal and hCG-induced secretion of progesterone and androstenedione at the highest doses, but did not affect basal secretion of estradiol or testosterone. IGF-II, at the highest doses, increased (P < 0.05) thecal steroidogenesis, but only after administration of hCG. In contrast, TGF.beta. increased (P < 0.05) basal and gonadotrophin-induced secretion of estradiol but inhibited thecal secretion of progesterone, androstenedione, and testosterone. EGF did not alter thecal secretion of progesterone, androstenedione, or testosterone but significantly (P < 0.05) inhibited basal and hCG-stimulated secretion of estradiol. In conclusion, insulin, IGF-I, IGF-II, EGF, and TGF.beta. can modulate steroidogenesis in porcine theca cells.