Phenotyping of plants in competitive but controlled environments: a study of drought response in transgenic wheat
Phenotyping of plants in competitive but controlled environments: a study of drought response in transgenic wheat
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DOI：
10.1071/fp16202
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发表时间：
2017-01-01
影响因子：
3
通讯作者：
Haefele, Stephan M.
中科院分区：
文献类型：
--
作者：
Kovalchuk, Nataliya;Laga, Hamid;Haefele, Stephan M.
近年来，人们对小麦改良新技术的兴趣大大增加。为了在更现实的田间条件下筛选转基因种质，我们开发了一个转基因小麦和大麦竞争生长的表型分析平台。在这项研究中，我们使用该平台（1）测试所选启动子和基因组合增加耐旱性的能力，（2）测试我们的平台筛选转基因植物在竞争中生长的性能的功能和能力，以及（3）开发和测试成像和分析过程作为获得额外的，在整个生长周期中对植物生长的非破坏性数据，而不是仅仅依赖于季节结束时的破坏性采样。结果表明，在充分灌溉条件下，几个转基因株系的生物量和/或粒重比野生型对照高，但只有一种情况下优势显着。在干旱胁迫下，没有转基因似乎显示出任何谷粒重量优势，并且只有两个品系具有比野生型显著但不显著更高的生物量重量。然而，它们在干旱胁迫下的评估由于其延迟的开花日期而处于不利地位，这增加了它们与野生型相比所经历的干旱胁迫。在本赛季连续成像提供了额外的和非破坏性的表型信息的冠层发展的小地块在我们的表型平台。每日冠层覆盖数据与收获指标的相关性分析表明，从冠层覆盖数据收获指标的最佳预测值是实现了从抽穗/开花到早熟的观测，而早季观测只有有限的诊断价值。在早期生长阶段的生物量/叶发育与生物量或谷物产量数据的相关性很小，这一结果对仅集中在早期发育阶段的成像方法提出了质疑。
In recent years, the interest in new technologies for wheat improvement has increased greatly. To screen genetically modified germplasm in conditions more realistic for a field situation we developed a phenotyping platform where transgenic wheat and barley are grown in competition. In this study, we used the platform to (1) test selected promoter and gene combinations for their capacity to increase drought tolerance, (2) test the function and power of our platform to screen the performance of transgenic plants growing in competition, and (3) develop and test an imaging and analysis process as a means of obtaining additional, non-destructive data on plant growth throughout the whole growth cycle instead of relying solely on destructive sampling at the end of the season. The results showed that several transgenic lines under well watered conditions had higher biomass and/or grain weight than the wild-type control but the advantage was significant in one case only. None of the transgenics seemed to show any grain weight advantage under drought stress and only two lines had a substantially but not significantly higher biomass weight than the wild type. However, their evaluation under drought stress was disadvantaged by their delayed flowering date, which increased the drought stress they experienced in comparison to the wild type. Continuous imaging during the season provided additional and non-destructive phenotyping information on the canopy development of mini-plots in our phenotyping platform. A correlation analysis of daily canopy coverage data with harvest metrics showed that the best predictive value from canopy coverage data for harvest metrics was achieved with observations from around heading/flowering to early ripening whereas early season observations had only a limited diagnostic value. The result that the biomass/leaf development in the early growth phase has little correlation with biomass or grain yield data questions imaging approaches concentrating only on the early development stage.