Polyethyleneimine is an effective permeabilizer of Gram-negative bacteria
Polyethyleneimine is an effective permeabilizer of Gram-negative bacteria
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DOI：
10.1099/00221287-143-10-3193
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发表时间：
1997-10-01
期刊：
影响因子：
--
通讯作者：
Koski, P
中科院分区：
文献类型：
--
作者：
Helander, IM;Alakomi, HL;Koski, P
以大肠埃希菌、铜绿假单胞菌和鼠伤寒沙门氏菌为靶菌，研究了聚阳离子聚乙烯亚胺(PEI)对革兰氏阴性菌外膜通透性的影响。在小于20微克毫升(-1)的浓度下，PEI增加了细菌对1-N-苯基萘胺的摄取，这是一种疏水探针，在脂类环境中其量子产率大大增加，表明PEI增加了对外膜的疏水渗透。PEI的效果与著名的渗透剂EDTA相当。毫摩尔浓度的氯化镁延缓了PEI的通透性，但不能完全抑制其通透性。PEI还增加了受试物种对疏水抗生素克林霉素、红霉素、岩藻素、新霉素和利福平的敏感性，而没有直接杀菌作用。在用PEI处理细菌的试验中，PEI使细菌对洗涤剂十二烷基硫酸钠的溶解作用敏感。在同时存在PEI和十二烷基硫酸钠的检测中，未观察到致敏作用，表明PEI和十二烷基硫酸钠相互灭活。此外，还观察到了非离子洗涤剂Triton X-100对铜绿假单胞菌的增敏作用。综上所述，PEI是一种有效的革兰氏阴性菌外膜渗透剂。
The effect of the polycation polyethyleneimine (PEI) on the permeability properties of the Gram-negative bacterial outer membrane was investigated using Escherichia coli, Pseudomonas aeruginosa and Salmonella typhimurium as target organisms. At concentrations of less than 20 mu g ml(-1), PEI increased the bacterial uptake of 1-N-phenyl naphthylamine, which is a hydrophobic probe whose quantum yield is greatly increased in a lipid environment, indicating increased hydrophobic permeation of the outer membrane by PEI. The effect of PEI was comparable to that brought about by the well-known permeabilizer EDTA. Permeabilization by PEI was retarded but not completely inhibited by millimolar concentrations of MgCl2. PEI also increased the susceptibility of the test species to the hydrophobic antibiotics clindamycin, erythromycin, fucidin, novobiocin and rifampicin, without being directly bactericidal. PEI sensitized the bacteria to the lytic action of the detergent SDS in assays where the bacteria were pretreated with PEI. In assays where PEI and SDS were simultaneously present, no sensitization was observed, indicating that PEI and SDS were inactivating each other. In addition, a sensitizing effect to the nonionic detergent Triton X-100 was observed for P. aeruginosa. In conclusion, PEI was shown to be a potent permeabilizer of the outer membrane of Gram-negative bacteria.