Revealing genetic relationships between compounds affecting boar taint and reproduction in pigs
Revealing genetic relationships between compounds affecting boar taint and reproduction in pigs
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DOI：
10.2527/jas.2010-3290
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发表时间：
2011-03-01
影响因子：
3.3
通讯作者：
Lien, S.
中科院分区：
文献类型：
--
作者：
Grindflek, E.;Meuwissen, T. H. E.;Lien, S.
野猪污染的特征是完好无损的公猪有令人不快的味道或气味，主要原因是雄酮和臭豆素浓度增加，其次是吲哚含量增加。野猪的污染化合物skatole和indole由肠道细菌产生，在肝脏中代谢，并储存在脂肪组织中。另一方面，雄酮在睾丸中与睾酮和雌激素一起合成，这是已知的影响生育的重要因素。这项研究的主要目的是调查主要的公猪污染化合物中涉及的遗传因素之间的关系，试图找到在不减少与繁殖相关的化合物的情况下减少公猪污染的方法。对与公猪污染(雄酮、雌酚、吲哚)和繁殖(睾酮、17β-雌二醇和雌酮硫酸酯)有关的化合物的遗传力和性状间的遗传相关性进行了估计。脂肪和血浆中雄激素浓度的遗传力都在0.47-0.67之间，而脂肪和吲哚的遗传力略低(0.27-0.41)。杜洛克和长白猪血浆和脂肪中雄烯酮的遗传相关极高(0.91~0.98)。此外，雄酮(血浆和脂肪)与其他性激素(硫酸雌酮、17β-雌二醇和睾酮)之间的遗传相关性非常高，在0.80到0.95的范围内。此外，对1,533个纯种长白和1,027个纯种杜洛克进行了全基因组关联研究(GWA)和连锁不平衡与连锁分析(LDLA)，以找出与公猪污染化合物雄酮、臭豆素和吲哚有关的基因组区域以及与生育性状相关的性激素。这两个品种包含多达3297个信息丰富的SNP标记，包括来自几个公猪污染候选基因的SNP。从GWA的研究中，我们发现共有27个区域在全基因组水平上显著(P<；0.05)，另外7个区域在染色体水平上显著。从LDLA研究中，7个区域在全基因组水平上显著，另外7个区域在染色体水平上显著。在第1、2、3、7、13和14号染色体上影响脂肪中脂肪脱氢表雄酮和吲哚的6个区域，6号染色体上只影响血浆中雄酮的1个区域，以及第3、4、13和15号染色体上影响雄酮、睾酮和雌激素的5个区域，得到了最令人信服的关联。
Boar taint is characterized by an unpleasant taste or odor in intact male pigs and is primarily attributed to increased concentrations of androstenone and skatole and to a lesser extent by increased indole. The boar taint compounds skatole and indole are produced by gut bacteria, metabolized in the liver, and stored in the fat tissue. Androstenone, on the other hand, is synthesized in the testis along with testosterone and estrogens, which are known to be important factors affecting fertility. The main goal of this study was to investigate the relationship between genetic factors involved in the primary boar taint compounds in an attempt to discover ways to reduce boar taint without decreasing fertility-related compounds. Heritabilities and genetic correlations between traits were estimated for compounds related to boar taint (androstenone, skatole, indole) and reproduction (testosterone, 17 beta-estradiol, and estrone sulfate). Heritabilities in the range of 0.47 to 0.67 were detected for androstenone concentrations in both fat and plasma, whereas those for skatole and indole were slightly less (0.27 to 0.41). The genetic correlations between androstenone in plasma and fat were extremely high (0.91 to 0.98) in Duroc and Landrace. In addition, genetic correlations between androstenone (both plasma and fat) and the other sex steroids (estrone sulfate, 17 beta-estradiol, and testosterone) were very high, in the range of 0.80 to 0.95. Furthermore, a genome-wide association study (GWA) and a combined linkage disequilibrium and linkage analysis (LDLA) were conducted on 1,533 purebred Landrace and 1,027 purebred Duroc to find genome regions involved in genetic control of the boar taint compounds androstenone, skatole, and indole, and sex hormones related to fertility traits. Up to 3,297 informative SNP markers were included for both breeds, including SNP from several boar taint candidate genes. From the GWA study, we found that altogether 27 regions were significant at a genome-wide level (P < 0.05) and an additional 7 regions were significant at a chromosomal level. From the LDLA study, 7 regions were significant on a genome-wide level and an additional 7 regions were significant at a chromosomal level. The most convincing associations were obtained in 6 regions affecting skatole and indole in fat on chromosomes 1, 2, 3, 7, 13, and 14, 1 region on chromosome 6 affecting androstenone in plasma only, and 5 regions on chromosomes 3, 4, 13, and 15 affecting androstenone, testosterone, and estrogens.