Effects of Polygonatum sibiricum rhizome ethanol extract in high-fat diet-fed mice
Effects of Polygonatum sibiricum rhizome ethanol extract in high-fat diet-fed mice
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DOI：
10.3109/13880209.2014.932393
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发表时间：
2015-04-01
影响因子：
3.8
通讯作者：
Kang, Jae-Hoon
中科院分区：
文献类型：
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作者：
Ko, Jong-Hee;Kwon, Hyuk-Sang;Kang, Jae-Hoon
背景：西伯利亚黄精（百合科）的根茎长期以来一直被用于治疗糖尿病相关并发症。然而，西伯利亚参对代谢紊乱的药理学机制尚不清楚。 目的：本研究研究西伯利亚参乙醇提取物（指定 ID1216）对高脂（HF）饮食喂养小鼠体重减轻等肥胖状况的影响，并探讨潜在的潜在机制。方法：确定西伯利亚参根茎的代谢影响 研究人员以 HF 饮食喂养的小鼠口服 ID1216，剂量为 250 和 1000 mg/kg/d，持续 10 周，并分析与代谢综合征相关的各种因素。我们还检测了 ID1216 对 3T3-L1 细胞中参与脂肪生成和脂肪分解的基因以及 C2C12 肌细胞中与能量稳态相关的基因表达的影响。结果：ID1216 给药导致体重增加 (37.5%)、脂肪组织中脂质积累 (52.8%) 和血浆水平显着降低。 与载体治疗组相比，250 mg/kg/d 剂量的甘油三酯（26.4%）和游离脂肪酸（28.1%）得到改善，胰岛素抵抗也得到改善。此外，ID1216被发现可以调节3T3-L1细胞中与脂肪生成和脂肪酸氧化相关的基因的表达，并增强C2C12肌细胞中调节能量稳态的基因的表达。结论：ID1216可能是一种有前途的治疗剂，通过sirtuin-1和过氧化物酶体增殖物激活受体g来改善肥胖状况。 辅激活因子-1a 途径。
Context: The rhizome of Polygonatum sibiricum Redoute (Liliaceae) has long been used to treat diabetes-associated complications. However, the pharmacological mechanism of P. sibiricum on metabolic disorders is not clear.Objective: This study investigates the effect of an ethanol extract of P. sibiricum rhizomes (designated ID1216) on obesity conditions including weight loss in high-fat (HF) diet-fed mice and explores the potential underlying mechanisms.Methods: To identify the metabolic impact of the P. sibiricum rhizome extract, HF diet-fed mice were administered ID1216 orally at doses of 250 and 1000 mg/kg/d for 10 weeks, and various factors related to metabolic syndrome were analyzed. We also examined the effects of ID1216 on the expression of genes involved in adipogenesis and lipolysis in 3T3-L1 cells, as well as genes associated with energy homeostasis in C2C12 myocytes.Results: ID1216 administration led to significant decreases in body weight gain (37.5%), lipid accumulation in adipose tissues (52.8%), and the levels of plasma triglycerides (26.4%) and free fatty acids (28.1%) at a dose of 250 mg/kg/d, compared with the vehicle-treated group, as well as improved insulin resistance. In addition, ID1216 was found to regulate the expression of genes related to adipogenesis and fatty acid oxidation in 3T3-L1 cells and enhance the expression of genes that modulate energy homeostasis in C2C12 myocytes.Conclusions: ID1216 may be a promising therapeutic agent for improving obesity conditions through the sirtuin-1 and peroxisome proliferator-activated receptor g coactivator-1a pathway.