Cell-Matrix Elastocapillary Interactions Drive Pressure-based Wetting of Cell Aggregates.
Cell-Matrix Elastocapillary Interactions Drive Pressure-based Wetting of Cell Aggregates.
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细胞-基质弹性毛细管相互作用驱动细胞聚集体的基于压力的润湿。
DOI：
10.1103/physrevx.12.031027
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Murrell,M
中科院分区：
文献类型：
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作者：
Yousafzai,MS;Yadav,V;Amiri,S;Staddon,MF;Errami,Y;Jaspard,G;Banerjee,S;Murrell,M
细胞表面张力引起的机械应力的大小可能与细胞长度尺度上基质的体弹性相当，但毛细效应如何影响组织形状和运动尚不清楚。在这项工作中，我们诱导细胞聚集体的润湿（扩散和迁移），作为活性液滴在不同弹性的粘附基质上的模型，并将润湿动力学与界面张力平衡联系起来。在润湿刚性衬底后，细胞-衬底张力驱动单层向外膨胀。相反，在湿润柔顺的衬底后，细胞-衬底张力减弱，毛细力聚集形成驱动膨胀的内部压力。因此，我们通过实验、数据驱动的建模和计算模拟表明，肌球蛋白驱动的“活性弹性毛细血管”效应能够使润湿机制适应底物刚性，并引入一种新的、基于压力的机制来指导细胞集体运动。
The magnitude of mechanical stresses caused by cell surface tension may be comparable to the bulk elasticity of their matrix on cellular length scales, yet how capillary effects influence tissue shape and motion are unknown. In this work, we induce wetting (spreading and migration) of cell aggregates, as models of active droplets onto adhesive substrates of varying elasticity, and correlate the dynamics of wetting to the balance of interfacial tensions. Upon wetting rigid substrates, cell-substrate tension drives outward expansion of the monolayer. By contrast, upon wetting compliant substrates, cell-substrate tension is attenuated and aggregate capillary forces contribute to internal pressures that drive expansion. Thus, we show by experiments, data-driven modeling, and computational simulations that myosin-driven “active elastocapillary” effects enable adaptation of wetting mechanisms to substrate rigidity and introduce a novel, pressure-based mechanism for guiding collective cell motion.