rpoD operon promoter used by sigma H-RNA polymerase in Bacillus subtilis.
rpoD operon promoter used by sigma H-RNA polymerase in Bacillus subtilis.
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枯草芽孢杆菌中 sigma H-RNA 聚合酶使用的 rpoD 操纵子启动子。
DOI：
10.1128/jb.170.4.1617-1621.1988
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发表时间：
1988
影响因子：
3.2
通讯作者：
MoranJr,CP
中科院分区：
文献类型：
--
作者：
Carter3rd,HL;Wang,LF;Doi,RH;MoranJr,CP
枯草芽孢杆菌sigA （rpoD）操纵子的三个启动子直接转录。启动子P1和P2在指数生长阶段使用，而P3仅在静止阶段使用。我们研究了这些启动子在启动子探针质粒中的使用情况，发现编码次级RNA聚合酶sigma因子sigma h的spoOH突变阻止了P3的表达。此外，我们发现含有sigma h的RNA聚合酶在体外有效而准确地使用P3启动子。显然，这种对指数生长至关重要的操纵子在孢子形成的早期阶段由这种次级形式的RNA聚合酶转录。将P3启动子与spoVG启动子（也被sigma H-RNA聚合酶使用）的核苷酸序列进行比较，发现这些启动子-10和-35区域的序列可能表明sigma H-RNA聚合酶对启动子的识别。
Three promoters direct transcription of the sigA (rpoD) operon in Bacillus subtilis. Promoters P1 and P2 are used during the exponential growth phase, whereas P3 is used only during the stationary phase. We examined the use of these promoters in promoter-probe plasmids and found that expression from P3 was prevented by a mutation in spoOH, which encodes the secondary RNA polymerase sigma factor sigma H. Moreover, we found that sigma H-containing RNA polymerase efficiently and accurately used the P3 promoter in vitro. Evidently, this operon, which is essential for exponential growth, is transcribed during the early phase of sporulation by this secondary form of RNA polymerase. Comparison of the nucleotide sequences of the P3 promoter and the spoVG promoter, which also is used by sigma H-RNA polymerase, revealed sequences at the -10 and -35 regions of these promoters that may signal recognition of promoters by sigma H-RNA polymerase.